Really smart bioconjugates of smart polymers and receptor proteins
Really smart bioconjugates of smart polymers and receptor proteins
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DOI：
10.1002/1097-4636(20001215)52:4
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发表时间：
2000-12-15
期刊：
影响因子：
--
通讯作者：
Miyata, T
中科院分区：
文献类型：
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作者：
Hoffman, AS;Stayton, PS;Miyata, T
在过去的18年里，我们一直致力于刺激反应聚合物系统的合成和应用，特别是聚合物-生物分子偶联物。本文总结了我们的工作与这些偶联系统之一，特别是聚合物-蛋白质偶联物。我们包括通过随机聚合物偶联到赖氨酸氨基而制备的偶联物，也包括通过特定位点的聚合物偶联到特定氨基酸位点而制备的偶联物，这些位点是通过基因工程进入蛋白质的已知氨基酸序列的。我们描述了酶和几种亲和识别蛋白的热敏随机偶联物的制备和性质。我们还用温度敏感聚合物、ph敏感聚合物和光敏聚合物制备了链霉亲和素的位点特异性共轭物。本文综述了这些共轭物的制备及其许多令人着迷的应用。(C) 2000 John Wiley & Sons, Inc。
Over the past 18 years we have been deeply involved with the synthesis and applications of stimuli-responsive polymer systems, especially polymer-biomolecule conjugates. This article summarizes our work with one of these conjugate systems, specifically polymer-protein conjugates. We include conjugates prepared by random polymer conjugation to lysine amino groups, and also those prepared by site-specific conjugation of the polymer to specific amino acid sites that are genetically engineered into the known amino acid sequence of the protein. We describe the preparation and properties of thermally sensitive random conjugates to enzymes and several affinity recognition proteins. We have also prepared site-specific conjugates to streptavidin with temperature-sensitive polymers, pH-sensitive polymers, and light-sensitive polymers. The preparation of these conjugates and their many fascinating applications are reviewed in this article. (C) 2000 John Wiley & Sons, Inc.