Chemical Structural and Chain Conformational Characterization of Some Bioactive Polysaccharides Isolated from Natural Sources
Chemical Structural and Chain Conformational Characterization of Some Bioactive Polysaccharides Isolated from Natural Sources
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DOI：
10.1002/chin.200933258
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
Liqun Yang;Li‐Ming Zhang
中科院分区：
文献类型：
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作者：
Liqun Yang;Li‐Ming Zhang
摘要从天然来源中分离得到的多糖具有多种重要的生物活性，如抗肿瘤、免疫调节和抗炎作用，这些活性与其化学结构和链构象密切相关。本文综述了从天然来源中分离的几种重要生物活性多糖的结构和构象特征。通过FTIR、液态NMR（一维和二维）、固态NMR、拉曼光谱、气相色谱（GC）、GC-MS（GC-MS）和高效液相色谱（HPLC）分析了化合物的化学结构。利用静态和动态光散射、基于稀溶液理论的粘度分析、圆二色性分析、原子力显微镜（AFM）（包括单分子AFM和基于AFM的单分子力谱）、荧光相关光谱和核磁共振光谱等方法研究了多糖在溶液中的链构象。
Abstract Some polysaccharides isolated from natural sources show various important biological activities, such as antitumor, immunomodulatory, and anti-inflammatory effects, which are strongly affected by their chemical structures and chain conformations. This article attempts to review the current development on structural and conformational characterization of some importantly bioactive polysaccharides isolated from natural sources. The chemical structures were analyzed by FTIR, liquid-state NMR (one and two dimensions), solid-sate NMR, Raman spectroscopy, gas chromatography (GC), GC–Mass (GC–MS), and high-performance liquid chromatography (HPLC). The chain conformations of polysaccharides in solutions were investigated using static and dynamic light scattering, viscosity analysis based on the theory of dilute polymer solution, circular dichroism analysis, atomic force microscopy (AFM) including single molecular AFM and AFM-based single-molecule force spectroscopy, fluorescence correlation spectroscopy and NMR spectroscopy.