REVERSAL OF LEFT-RIGHT ASYMMETRY - A SITUS-INVERSUS MUTATION
REVERSAL OF LEFT-RIGHT ASYMMETRY - A SITUS-INVERSUS MUTATION
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DOI：
10.1126/science.8480178
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发表时间：
1993-04-30
期刊：
影响因子：
56.9
通讯作者：
OVERBEEK, PA
中科院分区：
文献类型：
--
作者：
YOKOYAMA, T;COPELAND, NG;OVERBEEK, PA
在一个转基因小鼠家族中发现了隐性突变，该突变导致 100% 测试的纯合转基因小鼠的左右极性（反转）发生逆转。转基因整合位点侧翼的序列被克隆并定位到小鼠 4 号染色体上的 Tsha 和 Hxb 基因座之间。在早期胚胎发育过程中，植入后转向的方向（左右不对称的最早表现之一）在纯合转基因胚胎中发生了逆转。这种插入突变识别出控制胚胎转向和内脏左右极性的基因。
A recessive mutation was identified in a family of transgenic mice that resulted in a reversal of left-right polarity (situs inversus) in 100 percent of the homozygous transgenic mice tested. Sequences that flanked the transgenic integration site were cloned and mapped to mouse chromosome 4, between the Tsha and Hxb loci. During early embryonic development, the direction of postimplantation turning, one of the earliest manifestations of left-right asymmetry, was reversed in homozygous transgenic embryos. This insertional mutation identifies a gene that controls embryonic turning and visceral left-right polarity.