Enhanced targeting of ultrasound contrast agents using acoustic radiation force
Enhanced targeting of ultrasound contrast agents using acoustic radiation force
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DOI：
10.1016/j.ultrasmedbio.2007.01.005
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发表时间：
2007-07-01
影响因子：
2.9
通讯作者：
Hossack, John A.
中科院分区：
文献类型：
--
作者：
Rychak, Joshua J.;Klibanov, Alexander L.;Hossack, John A.
对比增强超声作为一种分子成像方式已显示出显著的前景。然而，一个潜在的缺点是超声造影剂（UCA）在实现与血管内皮上的靶分子的粘附方面可能具有的困难。微泡UCA在层流中表现出向血管轴的侧向迁移，阻止UCA与内皮接触。在目前的研究中，我们研究了低振幅声辐射作为一种机制，以移动循环UCA向靶向内皮细胞。采用活体显微镜观察P-选择素靶向微泡UCA在小鼠提睾肌微循环和股动脉血管中的滞留情况。声学治疗增强UCA保留P-选择素四倍，在提睾肌小静脉和股静脉和20倍，在股动脉。这些结果表明，声学治疗作为一种机制，使基于超声的分子成像在血管与血流动力学和解剖条件，否则对抗UCA保留。（电子邮件：jh7fj@virginia.edu）（C）2007年Elsevier Inc.出版世界超声医学与生物学联合会（World Federation for Ultrasound in Medicine & Biology）
Contrast-enhanced ultrasound has shown significant promise as a molecular imaging modality. However, one potential drawback is the difficulty that ultrasound contrast agents (UCA) may have in achieving adhesion to target molecules on the vascular endothelium. Microbubble UCA exhibit a lateral migration toward the vessel axis in laminar flow, preventing UCA contact with the endothelium. In the current study, we have investigated low-amplitude acoustic radiation as a mechanism to move circulating UCA toward targeted endothelium. Intravital microscopy was used to assess the retention of microbubble UCA targeted to P-selectin in the mouse cremaster microcirculation and femoral vessels. Acoustic treatment enhanced UCA retention to P-selectin four-fold in cremaster venules and in the femoral vein and 20-fold in the femoral artery. These results suggest acoustic treatment as a mechanism for enabling ultrasound-based molecular imaging in blood vessels with hemodynamic and anatomical conditions otherwise adversarial for UCA retention. (E-mail: jh7fj@virginia.edu) (C) 2007 Published by Elsevier Inc. on behalf of the World Federation for Ultrasound in Medicine & Biology.