ANTIVIRAL ACTIVITY OF A BILE PIGMENT, BILIVERDIN, AGAINST HUMAN HERPESVIRUS-6 (HHV-6) INVITRO
ANTIVIRAL ACTIVITY OF A BILE PIGMENT, BILIVERDIN, AGAINST HUMAN HERPESVIRUS-6 (HHV-6) INVITRO
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DOI：
10.1111/j.1348-0421.1992.tb02037.x
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发表时间：
1992-01-01
影响因子：
2.6
通讯作者：
YAMANISHI, K
中科院分区：
文献类型：
--
作者：
NAKAGAMI, T;TAJI, S;YAMANISHI, K
胆绿素（BV）是一种胆色素，在体外检测了其对人类疱疹病毒-6（HHV-6）的抗病毒活性。BV（10 μ g/ml）对MT-4细胞中HHV-6的复制有明显的抑制作用。吸附后处理细胞，其抗病毒作用减弱。在病毒感染后3小时用BV（40 μ g/ml）处理细胞对病毒复制没有抑制作用。在感染前用BV（10 μ g/ml）处理MT-4细胞也能显著抑制病毒复制，而BV（20 μ g/ml）不能灭活病毒体。BV的代谢产物胆红素和尿胆素对细胞内病毒复制有轻微的抑制作用。另一方面，BV对单纯疱疹病毒-1或人巨细胞病毒的复制没有有效的抑制活性。这些观察结果表明，BV可以与MT-4细胞相互作用，以病毒特异性方式抑制HHV-6感染的早期阶段。
Biliverdin (BV), a bile pigment, was examined for its antiviral activity against human herpesvirus-6 (HHV-6) in vitro. BV (10-mu-g/ml) markedly inhibited HHV-6 replication in MT-4 cells when the cells were treated during a virus adsorption period. Its antiviral effect was weakened when cells were treated after adsorption. Treatment of cells with BV (40-mu-g/ml) 3 hr after virus infection had no inhibitory effect on virus replication. Virus replication was also significantly inhibited by treatment of MT-4 cells with BV (10-mu-g/ml) before infection, while the virions were not inactivated by BV (20-mu-g/ml). Bilirubin and urobilin, metabolic derivatives of BV, showed slight inhibitory effects on virus replication in the cells. On the other hand, BV had no potent inhibitory activity in the replication of herpes simplex virus-1 or human cytomegalovirus. These observations suggest that BV could interact with MT-4 cells to inhibit an early stage of HHV-6 infection in a virus-specific manner.