A Mesh-Duox pathway regulates homeostasis in the insect gut.
A Mesh-Duox pathway regulates homeostasis in the insect gut.
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DOI：
10.1038/nmicrobiol.2017.20
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发表时间：
2017-03-01
影响因子：
28.3
通讯作者：
Cheng G
中科院分区：
文献类型：
--
作者：
Xiao X;Yang L;Pang X;Zhang R;Zhu Y;Wang P;Gao G;Cheng G
后生动物的肠道内有复杂的共生细菌群落。这些微生物的数量和质量随生理变化而动态波动。宿主对这种动态变化的反应和管理以及维持体内平衡的机制在很大程度上仍然未知。在这里，我们确定了一个双氧化酶（Duox）的调节途径，有助于维持埃及伊蚊和黑腹果蝇的肠道内稳态。我们发现，肠膜相关蛋白，命名为网格，在控制肠道细菌的增殖中起着重要的作用，通过调节Duox的表达，通过Arrestin介导的MAPK JNK/ERK磷酸化级联反应。Mesh和Duox的表达与肠道细菌微生物组相关，在蚊子中，在血餐后不久，肠道细菌微生物组急剧增加。补片消融消除了Duox诱导，导致肠道微生物组负荷增加。我们的研究揭示了Mesh介导的信号通路是昆虫肠道的一个中心稳态机制。
The metazoan gut harbors complex communities of commensal and symbiotic bacterial microbes. The quantity and quality of these microbes fluctuate dynamically in response to physiological changes. The mechanisms that hosts developed to respond to and manage such dynamic changes and maintain homeostasis remain largely unknown. Here, we identify a dual oxidase (Duox)-regulating pathway that contributes in maintaining homeostasis in the gut of both Aedes aegypti and Drosophila melanogaster. We show that a gut membrane-associated protein, named Mesh, plays an important role in controlling proliferation of gut bacteria by regulating Duox expression through an Arrestin-mediated MAPK JNK/ERK phosphorylation cascade. Expression of both Mesh and Duox is correlated with the gut bacterial microbiome that, in mosquitoes, increases dramatically soon after a blood meal. Ablation of Mesh abolishes Duox induction leading to an increase of the gut microbiome load. Our study reveals that the Mesh-mediated signaling pathway is a central homeostatic mechanism of the insect gut.