Phosphorylation of Rab proteins from the brain of Bombyx mori.
Phosphorylation of Rab proteins from the brain of Bombyx mori.
复制标题
家蚕大脑中 Rab 蛋白的磷酸化。
DOI：
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复制
发表时间：
2004
影响因子：
2.2
通讯作者：
Chisato Katsurauma
中科院分区：
文献类型：
--
作者：
T. Uno;Atsushi Nakao;Chisato Katsurauma
Rab蛋白在膜运输的调节中起重要作用。在此之前，我们从家蚕脑中分离出两个cDNA克隆（BRab 1和BRab 14），每个克隆编码Rab-protein家族的不同成员，并在大肠杆菌中表达，并使用亲和层析纯化。本研究从B脑cDNA文库中分离到一个cDNA克隆（BRab 8）。桑。重组蛋白在E. coli中进行纯化。接下来，在体外使用哺乳动物蛋白激酶检查这三种纯化的BRab蛋白的磷酸化。蛋白激酶C（PKC）磷酸化BRab8和BRab14蛋白。蛋白激酶A微弱磷酸化BRab8和BRab14蛋白。钙/钙调蛋白依赖性蛋白激酶微弱磷酸化BRab8蛋白。接下来是B的大脑。将桑细胞解剖并均质化。匀浆显示BRab8和BRab14蛋白的钙依赖性蛋白激酶活性。所以B大脑中的PKC。通过DEAE-Cellulofine层析和亲和层析对桑色素进行部分纯化。这种PKC磷酸化BRab 8和BRab 14蛋白。这些结果提示，RaB蛋白在B脑中的功能可能与RaB蛋白的表达有关。Mori受钙依赖性蛋白激酶调节。
Rab proteins play fundamental roles in the regulation of membrane traffic. Previously, from the brain of Bombyx mori we isolated two cDNA clones (BRab1 and BRab14), each of which encoded a different member of Rab-protein family and was expressed in Escherichia coli and purified using an affinity chromatography. In this study, one cDNA clone (BRab8) was isolated from a cDNA library from the brain of B. mori. The recombinant protein was expressed in E. coli and purified. Next, the phosphorylations of these three purified BRab proteins were examined, using mammalian protein kinases in vitro. Protein kinase C (PKC) phosphorylated BRab8 and BRab14 proteins. Protein kinase A faintly phosphorylated BRab8 and BRab14 proteins. Calcium/calmodulin-dependent protein kinase faintly phosphorylated BRab8 protein. Next, brains of B. mori were dissected and homogenized. The homogenate showed a calcium-dependent protein kinase activity of BRab8 and BRab14 proteins. So PKC from the brain of B. mori was partially purified by a sequence of chromatographies on DEAE-Cellulofine and affinity chromatography. This PKC phosphorylated BRab8 and BRab14 proteins. These results suggest that the function of Rab proteins in the brain of B. mori is regulated by calcium-dependent protein kinases.
在其活性形式下，GTP 结合蛋白 rab8 与应激激活蛋白激酶相互作用。
DOI：
10.1073/pnas.93.10.5151
发表时间：
1996
影响因子：
11.1
作者：
Ren,M;Zeng,J;DeLemos-Chiarandini,C;Rosenfeld,M;Adesnik,M;Sabatini,DD
通讯作者：
Sabatini,DD
DOI：
10.1042/bj3420353
发表时间：
1999-09
期刊：
The Biochemical journal
影响因子：
--
作者：
Michael L. Fitzgerald;Guy L. Reed
通讯作者：
Michael L. Fitzgerald;Guy L. Reed
rab15，一种在大鼠脑中特异性表达的新型低分子量 GTP 结合蛋白。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Elferink,LA;Anzai,K;Scheller,RH
通讯作者：
Scheller,RH