GAMMA-AMINOBUTYRIC ACID PATHWAYS IN THE CEREBELLUM STUDIED BY RETROGRADE AND ANTEROGRADE TRANSPORT OF GLUTAMIC-ACID DECARBOXYLASE ANTIBODY AFTER INVIVO INJECTIONS
GAMMA-AMINOBUTYRIC ACID PATHWAYS IN THE CEREBELLUM STUDIED BY RETROGRADE AND ANTEROGRADE TRANSPORT OF GLUTAMIC-ACID DECARBOXYLASE ANTIBODY AFTER INVIVO INJECTIONS
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DOI：
10.1007/bf00315638
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
WU, JY
中科院分区：
文献类型：
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作者：
CHANPALAY, V;PALAY, SL;WU, JY
将抗谷氨酸脱羧酶（GAD）（负责合成GABA的酶）的特征性抗体注射到[大鼠]小脑中。小皮质注射抗GAD抗体产生标记的星状，篮状，浦肯野和高尔基体细胞和它们的过程中的注射部位。GAD抗原抗体复合物在浦肯野细胞轴突中的顺行运输引起小脑深部和几个前庭核中的终末的强烈标记。在小脑深核内的少数多形性神经元中，有少量苔藓状纤维团标记并产生逆行标记和GAD免疫反应。注射到齿状核产生逆行标记浦肯野细胞体和顺行标记在少数苔藓纤维的roadlets。所有的预测符合先前报道的地形分布的皮质核和核皮质小脑通路。大多数浦肯野细胞深层核连接的GABA含量得到证实，并提供了新的证据，GABA成分的一部分，在小脑的核皮质通路。通过注射免疫前兔血清作为GAD抗体的替代物进行特异性免疫细胞化学对照。在这些病例中仅获得非特异性标记。秋水仙碱引起的累积增强GAD免疫反应性在所有情况下。体外抗体注射的方法可用于研究神经组织中的化学特异性连接。
Injections of characterized antibody against glutamic acid decarboxylase (GAD), the enzyme responsible for the synthesis of GABA, were made into the cerebellum [of rats]. Small cortical injections of anti-GAD antibody produced labeled stellate, basket, Purkinje and Golgi cells and their processes at the injection site. Anterograde transport of GAD antigen-antibody complexes in Purkinje cell axons caused intense labeling of terminals in deep cerebellar and several vestibular nuclei. Small groups of mossy fiber rosettes labeled and produced retrograde labeling and GAD immunoreactivity in a small number of pleomorphic neurons in the deep cerebellar nuclei. Injections into the dentate nucleus produced retrograde labeling in Purkinje cell bodies and anterograde label in a small number of mossy fiber rosettes. All projections conformed to previously reported topographic distributions of corticonuclear and nucleocortical cerebellar pathways. The GABA content of most Purkinje cell-deep nuclei connections was confirmed and new evidence was provided for a GABA component in part of the nucleo cortical pathway in the cerebellum. Immunocytochemical controls for specificity were conducted by injections of preimmune rabbit serum as a substitute for GAD antibody. Only nonspecific labeling was obtained in these cases. Colchicine caused a cumulative enhancement of GAD immunoreactivity in all cases. The method of in vitro antibody injections may be utilized to study chemically specific connections in nervous tissue.