Granular pneumocytes in primary culture secrete several major components of the extracellular matrix.
Granular pneumocytes in primary culture secrete several major components of the extracellular matrix.
复制标题
原代培养物中的颗粒性肺细胞分泌细胞外基质的几种主要成分。
DOI：
10.1021/bi00278a015
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Fisher,AB
中科院分区：
文献类型：
--
作者：
Sage,H;Farin,FM;Striker,GE;Fisher,AB
Helene Sage，* Federico M.作者：Farin，加里E.前锋和阿伦B。Fisher摘要：大鼠肺泡II型上皮细胞（颗粒肺细胞）的原代培养物产生肺细胞外基质的几种成分。分泌到培养基中的放射性标记的蛋白质的离子交换色谱法分馏导致这些组分中的两个的部分纯化：纤连蛋白和IV型前胶原。这些蛋白质的鉴定十二烷基硫酸钠-聚丙烯酰胺凝胶电泳证实了放射免疫沉淀研究与亲和纯化的抗体。血小板反应蛋白是一种血小板α-颗粒蛋白，最近发现可由内皮细胞和其他间质细胞分泌，并可能参与血小板聚集。此外，还通过从二乙氨基乙基纤维素中洗脱纯化了血小板反应蛋白。在成人肺中已描述了超过40种不同的细胞类型[综述见Kuhn（1976）]。这种细胞异质性不仅反映了整个肺结构的结构多样性，还反映了该器官的代谢和功能特性。近年来，随着人们对一次气体交换过程认识的加深，
Helene Sage,* Federico M. Farin, Gary E. Striker, and Aron B. Fisher abstract: Primary cultures of rat alveolar type II epithelial cells (granular pneumocytes) producedseveral components of the pulmonaryextracellular matrix. Fractionation by ion-exchange chromatography of radiolabeled protein secreted into the culture medium resulted in the partial purification of two of these components: fibronectin and type IV procollagen. Identification of these proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was confirmed by radioimmune-precipitation studies with affinity-purified antibodies. Thrombospondin, a platelet a-granule protein that was recently shown to be secreted by endothelial and other mesenchymally derived cells and may be involved in platelet aggregation, was, in addition, purified by elution from diethylaminoethylcelluloseIVIore than 40 different cell types have been described in adult human lung [for a review, see Kuhn (1976)]. This cellular heterogeneity reflects not only the structural diversity of the lung architecture in toto but also the metabolic and functional properties of this organ as well. In recentyears, an increased understanding of the primary gas-exchanging