Separation and analysis of the glycoform populations of ribonuclease B using capillary electrophoresis
Separation and analysis of the glycoform populations of ribonuclease B using capillary electrophoresis
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使用毛细管电泳分离和分析核糖核酸酶 B 的糖型群体
DOI：
10.1007/bf00731704
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发表时间：
1992
影响因子：
3
通讯作者：
R. Dwek
中科院分区：
文献类型：
--
作者：
P. Rudd;I. Scragg;E. Coghill;R. Dwek
如果要更详细地了解糖蛋白的结构、功能和加工过程，就必须开发分离、分析和监测糖型群体变化的方法。在这项研究中，完整的核糖核酸酶B被解析为五个群体的硼酸盐毛细管电泳根据特定的寡聚甘露糖结构与每个糖型。这些群体的相对比例与间接获得的百分比通过使用Bio-Gel P-4凝胶过滤，基质辅助激光解吸质谱和高效阴离子交换色谱法分析肼释放的寡糖。核糖核酸酶B与A消化过程中糖型群体组成的改变。用毛细管电泳（CE）法测定α（1-2）甘露糖苷酶。通过阴离子交换色谱法监测，在相同条件下消化游离低聚糖，发现速率存在差异，从而对低聚糖加工过程中蛋白质的作用有了一些了解。与其他方法结合，CE的这种新应用可能会被证明是对糖型群体研究可用技术的有用补充。
The development of methods to separate, analyse and monitor changes in glycoform populations is essential if a more detailed understanding of the structure, function and processing of glycoproteins is to emerge. In this study, intact ribonuclease B was resolved by borate capillary electrophoresis into five populations according to the particular oligomnnose structure associated with each glycoform. The relative proportions of these populations are correlated with the percentages obtained indirectly by analysis of the hydrazine released oligosaccharides using Bio-Gel P-4 gel filtration, matrix assisted laser desorption mass spectrometry and high performance anion exchange chromatography. Alterations in the composition of the glycoform populations during digestion of ribonuclease B withA. saitoi α(1–2)mannosidase were monitored by capillary electrophoresis (CE). Digestion of the free oligosaccharides under the same conditions, monitored by anion exchange chromatography, revealed a difference in rate, allowing some insight into the role of the protein during oligosaccharide processing. In conjunction with other methods, this novel application of CE may prove a useful addition to the techniques available for the study of glycoform populations.
通过高效阴离子交换色谱法分离酵母天冬酰胺连接的寡糖。
影响因子：
3.1
作者：
Hernandez,LM;Ballou,L;Ballou,CE
通讯作者：
Ballou,CE