Genome-Wide Analysis of mRNA Splicing vaRiants in Higher Plants
Genome-Wide Analysis of mRNA Splicing vaRiants in Higher Plants
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DOI：
10.35248/2376-0354.19.06.255
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发表时间：
2019
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影响因子：
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通讯作者：
Ying Li;Qianhuan Guo;C. Zheng;Shizhong Zhang;K. Yan
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文献类型：
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作者：
Ying Li;Qianhuan Guo;C. Zheng;Shizhong Zhang;K. Yan
选择性剪接 (AS) 从单个前体转录本产生多个 mRNA 剪接变体。最近的基因组测序分析和越来越多的开花植物实验证据表明，AS 比之前认为的更为普遍，并且在基因调控的多样化中发挥着至关重要的作用。尽管进行了大量研究，但从全球角度来看，植物中 mRNA 变异的程度和复杂性仍然很少被描述。在本研究中，研究了来自 12 个植物物种的 442,541 个注释基因的 589,034 个 mRNA 变体。所有AS基因根据mRNA变体的数量分为四组，即2V（两个变体）、3V（三个变体）、4V（四个变体）和5V+（五个或更多变体）。有趣的是，我们的分析表明，超过 50% 的 AS 基因在高等植物中仅产生两种变异。对基因结构的整体分析表明，随着 mRNA 变体数量的增加，AS 基因包含更多但更短的外显子和内含子。结果还表明，AS 对转录组和蛋白质组多样性的改善产生不同的影响。总而言之，跨物种分析提供了迄今为止在高等植物中最全面的注释剪接变体集，并扩展了当前关于 mRNA 变体的观点。
Alternative splicing (AS) produces multiple mRNA splicing variants from a single precursor transcript. Recent genome sequencing analyses and increasing experimental evidence in flowering plants have revealed that AS is far more prevalent than previously thought and plays crucial roles in the diversification of gene regulation. Despite numerous studies, the extent and complexity of mRNA variants in plants remain poorly characterized from a global perspective. In present study, 589,034 mRNA variants from 442,541 annotated genes of 12 plant species were investigated. All AS genes were classified into four groups on the basis of the numbers of mRNA variants, namely, 2V (two variants), 3V (three variants), 4V (four variants), and 5V+ (five or more variants). Interestingly, our analysis indicated that more than 50% AS genes generated only two variants in higher plants. A global analysis of gene structure revealed that AS genes contained more but shorter exons and introns as the number of mRNA variants increased. The results also suggested that AS elicited different effects on the improvement of transcriptome and proteome diversity. Taken together, cross-species analysis provided the most comprehensive set of annotated splicing variants in higher plants thus far and extended the current view about mRNA variants.