L-[4-C-11]-LABELED ASPARTIC-ACID - ENZYMATIC-SYNTHESIS, MYOCARDIAL UPTAKE, AND METABOLISM
L-[4-C-11]-LABELED ASPARTIC-ACID - ENZYMATIC-SYNTHESIS, MYOCARDIAL UPTAKE, AND METABOLISM
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DOI：
10.1021/jm00343a020
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发表时间：
1982-01-01
影响因子：
7.3
通讯作者：
BAUMGARTNER, FJ
中科院分区：
文献类型：
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作者：
BARRIO, JR;EGBERT, JE;BAUMGARTNER, FJ
无菌，无热原L-[4- 11 C]天冬氨酸制备11 CO2使用磷酸烯醇式丙酮酸羧化酶和谷氨酸/草酰乙酸转氨酶固定在琼脂糖支持，以确定它是否是一个有用的指示剂，在体内，心肌[能量]代谢的非侵入性测定。冠状动脉内推注L-[4- 11 C]天冬氨酸进入犬心肌显示三指数清除曲线，注射后100 s产生最大11 CO2。心肌转氨酶活性的失活修改示踪剂清除率和抑制11 CO2的产生。正电子计算机断层显像显示，静脉注射L-[4- 11 C]天冬氨酸后，恒河猴心肌中保留的11 C活性高于狗心肌中观察到的活性。心肌酶促转氨作用后，L-天冬氨酸的碳骨架可快速并入三羧酸循环，L-[4- 11 C]天冬氨酸可用于体内非侵入性评估局部心肌代谢。
Sterile, pyrogen-free L-[4-11C]aspartic acid was prepared from 11CO2 using phosphoenolpyruvate carboxylase and glutamic/oxaloacetic acid transaminase immobilized on Sepharose supports to determine if it is a useful indicator for in vivo, noninvasive determination of myocardial [energy] metabolism. An intracoronary bolus injection of L-[4-11C]aspartic acid into dog myocardium showed a triexponential clearance curve with maximal production of 11CO2 100 s after injection. Inactivation of myocardial transaminase activity modified the tracer clearance and inhibited the production of 11CO2. Positron-computed tomography imaging showed that the 11C activities retained in rhesus monkey myocardium are higher than those observed in dog heart after i.v. injection of L-[4-11C]aspartic acid. The rapid incorporation of the carbon skeleton of L-aspartic acid into the tricarboxylic acid cycle after enzymatic transamination in myocardium was demonstrated and L-[4-11C]aspartic acid could be of value for in vivo, noninvasive assessment of local myocardial metabolism.