Ultrasonic Scission of Deoxyribonucleic Acid in Aqueous Solution I. Conditions for Sonication and Molecular Weights of Sonicated Samples
Ultrasonic Scission of Deoxyribonucleic Acid in Aqueous Solution I. Conditions for Sonication and Molecular Weights of Sonicated Samples
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水溶液中脱氧核糖核酸的超声断裂 I. 超声处理条件和超声处理样品的分子量
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
O. Yamamoto
中科院分区：
文献类型：
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作者：
K. Fukudome;K. Yamaoka;Kenichiro Nishikori;Takamichi Takahashi;O. Yamamoto
通过在0 ℃下对小牛胸腺脱氧核糖核酸（DNA）水溶液（2 mg ml−1，含0.2 M NaCl）照射超声波（20 kHz），分子量（Mw）从8×106降低至约100 μ g/ml。2×105，无任何明显变性。通过改变超声能量水平，研究了超声处理DNA的分子量对辐照强度的依赖性。在25 ~ 105 W范围内，随着超声功率的增加，聚合物的分子量显著降低，在100-200 W范围内，聚合物的分子量达到稳定值（Mw=2.4×105）。
By irradiating ultrasonic wave (20 kHz).to calf thymus deoxyribonucleic acid (DNA) in aqueous solution (2 mg ml−1 containing 0.2 M NaCl) at 0°C, the molecular weight (Mw) was degraded from 8×106 to ca. 2×105 without any noticeable denaturation. The dependence of the molecular weight of sonicated DNA on the irradiation intensity was examined by varying ultrasonic energy levels. The molecular weights were remarkably decreased with the increase in ultrasonic power from 25 to 105 W, and reached a steady value (Mw=2.4×105) at 100—200 W.