Primer‐template specificity of DNA polymerases bound to mitochondria and nuclei
Primer‐template specificity of DNA polymerases bound to mitochondria and nuclei
复制标题
与线粒体结合的 DNA 聚合酶的引物模板特异性
DOI：
10.1016/0014-5793(73)80092-4
复制
发表时间：
1973
期刊：
影响因子：
3.5
通讯作者：
Y. Croisille
中科院分区：
文献类型：
--
作者：
L. Soriano;Y. Croisille
Recently DNA polymerases of eukaryotes have been the subject of intensive investigation as a consequence of the importance of research on RNA-dependent DNA polymerases in normal [1, 21 and in tumor cells and tissues [3-61. Attention has also been focused on the activity of DNA polymerases in regenerating tissues [7] and in developing systems [8-l 11. Margolis [121 has studied the distribution patterns of DNA-dependent DNA polymerase in different regions of brain of chick embryo, and one of us has shown [131 that mitochondria and nuclei of chick embryonic brain contain bound enzymes which catalyze the polymerization of dGMP when poly dG: poly dC or poly dG: poly rC are provided as primer templates.In the present paper we report the characterization of partially purified poly dT: poly rA-dependent DNA polymerase (bound to nuclei) and oligo dT: poly rA-dependent DNA polymerase (bound to mitochondria), both of them polymerizing thymidylic acid. Moreover, we have studied poly dG: poly dC and poly dG: poly rC-dependent activities polymerizing deoxyguanylic acid, in both mitochondria and nuclei.
