MicroRNA-mRNA expression profiles and functional network of submandibular gland in type 2 diabetic db/db mice
MicroRNA-mRNA expression profiles and functional network of submandibular gland in type 2 diabetic db/db mice
复制标题
2型糖尿病db/db小鼠下颌下腺MicroRNA-mRNA表达谱及功能网络
DOI：
10.1016/j.archoralbio.2020.104947
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发表时间：
2020-12-01
影响因子：
3
通讯作者：
Xiang, Ruo-Lan
中科院分区：
文献类型：
--
作者：
Liu, Hui-Min;Huang, Yan;Xiang, Ruo-Lan
目的：唾液分泌减少是糖尿病的常见症状。虽然microRNAs（miRNAs）在糖尿病的发病机制中发挥作用，但miRNAs对糖尿病唾液腺的具体作用尚不清楚。设计：我们采用高通量技术筛选db/db小鼠和db/m小鼠颌下腺（SMG）组织中差异表达（DE）的miRNAs和mRNAs。结果：在db/db小鼠和db/m小鼠SMG组织中共检测到28种DE miRNAs和1146种DE mRNAs。基因本体（GO）分析和京都基因与基因组百科全书（KEGG）通路分析表明，DE miRNAs与多种生物学过程和信号通路相关的术语高度相关。在相关通路中，紧密连接通路、自噬通路和转化生长因子-β（TGF-β）信号通路是值得注意的。AKT丝氨酸/苏氨酸激酶3（AKT 3）和磷酸肌醇-3激酶催化亚基δ（PIK 3CD）在糖尿病介导的少涎形成中也可能起重要作用。结论：本研究描述了db/db小鼠SMG组织中miRNA-mRNA表达谱和miRNA-mRNA网络。这些结果为糖尿病引起的唾液分泌减少提供了可能的分子机制，并为进一步研究提供了信息。
Objective: Hyposalivation is a common symptom of diabetes. Although microRNAs (miRNAs) play a role in the pathogenesis of diabetes, the specific effects of miRNAs on diabetic salivary glands are not clear.Design: We used high-throughput technologies to screen differentially expressed (DE) miRNAs and mRNAs in submandibular gland (SMG) tissues from db/db mice and db/m mice. DE miRNAs and mRNAs were confirmed using quantitative real-time polymerase chain reaction (qRT-PCR).Results: Twenty-eight DE miRNAs and 1146 DE mRNAs were identified between the SMG tissues of db/db mice and db/m mice. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that the DE miRNAs were highly associated with terms related to diverse biological processes and signalling pathways. Of the related pathways, the tight junction pathway, autophagy pathway and transforming growth factor-beta (TGF-beta) signalling pathway were notable. AKT serine/threonine kinase 3 (AKT3) and phosphoinositide-3 kinase catalytic subunit delta (PIK3CD) may also play important roles in the development of diabetes-mediated hyposalivation.Conclusions: Our research described the miRNA-mRNA expression profiles and miRNA-mRNA network in the SMG tissues of db/db mice. These results provide possible molecular mechanisms of diabetes-induced hyposalivation and information for further studies.