Comparison of intramyocardial and intravenous routes of delivering bone marrow cells for the treatment of ischemic heart disease: An experimental study
Comparison of intramyocardial and intravenous routes of delivering bone marrow cells for the treatment of ischemic heart disease: An experimental study
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DOI：
10.3727/000000004783983558
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Hamano, K
中科院分区：
文献类型：
--
作者：
Hayashi, M;Li, TS;Hamano, K
心肌梗死后骨髓细胞植入缺血性心脏可诱导血管生成，改善心功能。我们比较了心内和静脉输送bmc的优势。采用左前降支结扎法建立雌性黑鼠急性心肌梗死模型。老鼠被随机分为四组:给定一个心肌内的注射磷酸盐(PBS组),一个给定的静脉注射2 x 10(7)的bmc雄性老鼠(第四组),一个给定的心肌内的注入,总2 x 10(7)的bmc雄性老鼠在梗死区4分(IM组),和一个给定数量的10倍的bmc的静脉注射雄性老鼠(10十四组)。实时荧光定量PCR定量分析SRY基因显示，IM组梗死区BMCs的存活率在治疗后3 d显著高于IV和10xIV组（p < 0.05），但此后无显著差异。治疗14 d后，IM和10xIV组梗死心肌血流明显优于PBS和IV组（p < 0.05）。超声心动图显示PBS和IV组LVEF持续下降，而IM和10xIV组3天后趋于稳定。治疗后14 d， IM和10xIV组LVEF显著高于PBS和IV组（p < 0.01）。我们的研究结果表明，在诱导血管生成和修复损伤心肌方面，心肌内给药比静脉给药更有效。
The implantation of bone marrow cells (BMCs) into ischemic heart after myocardial infarction can induce angiogenesis and improve heart function. We compared the advantages of delivering BMCs intramyocardially and intravenously. An acute myocardial infarction model was created by the ligation of left anterior descending artery in female Dark Agouti rats. The rats were then randomly divided into four treatment groups: one given an intramyocardial injection of phosphate-buffered saline (PBS group), one given an intravenous injection of 2 x 10(7) BMCs from male rats (IV group), one given an intramyocardial injection with total of 2 x 10(7) BMCs from male rats at four points in the infarction area (IM group), and one given an intravenous injection of 10-fold the number of BMCs from male rats (10xIV group). Quantitative analysis of the SRY gene by real-time PCR showed that the survival of BMCs in the infarcted area was significantly higher in the IM group than in the IV and 10xIV groups, 3 days after treatment (p < 0.05), but not thereafter. However, the blood flow in the infarcted myocardium was significantly better in the IM and 10xIV groups than in the PBS and IV groups 14 days after treatment (p < 0.05). Echocardiography showed that the LVEF continued to decrease in the PBS and IV groups, but was stable after 3 days in the IM and 10xIV groups. By 14 days after treatment, the LVEF was significantly higher in the IM and 10xIV groups than in the PBS and IV groups (p < 0.01). Our results showed that BMCs were more effective delivered intramyocardially than intravenously for inducing angiogenesis and repairing injured myocardium.