AN INVIVO MODEL TO QUANTIFY MOTOR AND SENSORY PERIPHERAL-NERVE REGENERATION USING BIORESORBABLE NERVE GUIDE TUBES
AN INVIVO MODEL TO QUANTIFY MOTOR AND SENSORY PERIPHERAL-NERVE REGENERATION USING BIORESORBABLE NERVE GUIDE TUBES
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DOI：
10.1016/0006-8993(85)91130-8
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
SIDMAN, RL
中科院分区：
文献类型：
--
作者：
DASILVA, CF;MADISON, R;SIDMAN, RL
为了研究运动和感觉神经轴突再生的速度和时间过程，提出了一种体内制备方法。将切断的坐骨神经的断端插入5~6 mm的无毒可生物吸收神经导管的两端，在成年小鼠身上形成4 mm的神经间隙。随后，用辣根过氧化物酶(HRP)逆行标记横跨间隙再生轴突的脊髓腹侧和L3-L5背根节的细胞体。HRP应用于神经导向器远端3 mm处，只有通过神经导向器再生的轴突才能接触到。分别于神经切断后2、4、6周取40μm连续切片进行标记细胞计数。结果表明，随着时间的推移，标记的运动和感觉细胞体的数量显著增加。在横断后6周，腹角运动细胞和背根节感觉细胞的数量约为对照组的三分之二和三分之一。这些数据作为比较添加剂对神经导管腔在感觉和运动神经元反应方面的不同影响的基线。
An in vivo preparation is presented to study the rate and time course of motor and sensory axonal regeneration. The cut ends of a transected sciatic nerve were inserted into each end of a 5–6 mm non-toxic and bioresorbable nerve guide tube to create a 4 mm nerve gap in adult mice. Subsequently, cell bodies in the ventral spinal cord and L3–L5 dorsal root ganglia that had regenerated axons across the gap were retrogradely labeled with horseradish peroxidase (HRP). The HRP was applied 3 mm distal to the nerve guide and was accessible only to axons that had regenerated through the nerve guide. Labeled cells were counted in 40 μm serial sections at 2, 4 and 6 weeks after initial nerve transection. The results indicate a significant increase in the number of labeled motor and sensory cell bodies over time. By 6 weeks after transection, approximately two thirds as many ventral horn motor cells and one third as many dorsal root ganglion sensory cells were labeled as in control non-transected animals. These data serve as a baseline to compare differential effects of additives to the nerve guide lumen in terms of sensory and motor neuron response.