WHERE DOES DAMAGE LEAD TO ENHANCED FOOD AVERSION - THE VENTRAL PALLIDUM SUBSTANTIA INNOMINATA OR LATERAL HYPOTHALAMUS
WHERE DOES DAMAGE LEAD TO ENHANCED FOOD AVERSION - THE VENTRAL PALLIDUM SUBSTANTIA INNOMINATA OR LATERAL HYPOTHALAMUS
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DOI：
10.1016/0006-8993(93)90053-p
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发表时间：
1993-10-08
期刊：
影响因子：
2.9
通讯作者：
BERRIDGE, KC
中科院分区：
文献类型：
--
作者：
CROMWELL, HC;BERRIDGE, KC
众所周知，外侧下丘脑（LH）的病变会导致失语症。先前的几项研究报道，外侧下丘脑损伤除了产生失语症外，还会产生食物厌恶。然而，LH附近其他区域的损伤也会产生失语和增强的厌恶感。本研究的目的是确定引起厌恶的病变的位置。将小的双侧兴奋毒素病变（QUIN， 10杯1例或IBO， 15杯1例）或双侧假注射到白球（GP）、腹侧白球/ nominata （VP/SI）或外侧下丘脑（LH）。通过慢性口腔插管将蔗糖溶液（1 M）注入口腔，引起对味觉的情感反应。对厌恶反应（打哈欠、摩擦下巴、摇头和前肢连枷）的数量进行了统计。我们绘制了单个损伤的分布图，并确定了单个损伤诱发厌恶的“必要和充分”部位（所有表现出增强厌恶反应的大鼠共有的重叠损伤区域）。为了识别病变，使用了两种病变映射技术：(1)传统的神经元计数程序，其中试图对大脑区域内的所有神经元进行计数；(2)一种新的改进的“分数法”程序，包括在大脑区域内的点位置进行详尽的400倍放大计数。结果表明，对食物的厌恶反应仅在双侧神经元（bbb70 %）从尾侧腹内侧VP/SI单独丢失后才会增强。这一共有部位外侧直径1.0 mm，背腹侧直径0.5 mm，背腹侧直径1.0 mm。局限于LH的损伤即使产生失语症也不会产生增强的厌恶感。厌恶的关键区域位于苍白球的腹侧和内侧以及外侧下丘脑的背侧和外侧。
It is well known that lesions of the lateral hypothalamus (LH) produce aphagia. Several previous studies have reported that lateral hypothalamus damage produces food aversion in addition to aphagia. However, damage to other regions near the LH also produce aphagia and enhanced aversion. The purpose of this study was to resolve where the site or sites for aversion-inducing lesions is/are located. Small, bilateral excitotoxin lesions (QUIN, 10 mug in 1 mul or IBO, 15 mug in 1 mul) or bilateral sham injections of vehicle were made into the globus pallidus (GP), the ventral pallidum/substantia innominata (VP/SI) or the lateral hypothalamus (LH). Affective reactions to taste were elicited by infusing sucrose solutions (1 M) into the mouth via chronic oral cannulae. The number of aversive responses (gapes, chin-rubbing, head-shaking and forelimb flails) emitted was tallied. Individual lesions were mapped and a single 'necessary and sufficient' site for damage-induced aversion was identified (the area of overlapping damage common to all rats that showed enhanced aversive reactions). To identify the lesions, two lesion-mapping techniques were used: (1) a conventional neuron-counting procedure in which an attempt is made to count all neurons within a brain region, and (2) a new modified 'fractionator' procedure consisting of exhaustive 400x magnification counts at point locations within a brain region. Results indicated that aversive reactions to food are enhanced only following bilateral neuron loss (> 70%) from the caudal ventromedial VP/SI alone. This shared site has a lateral diameter of 1.0 mm, a dorsoventral diameter of 0.5 mm and a rostrocaudal diameter of 1.0 mm. Damage restricted to the LH never produced enhanced aversion even when it produced aphagia. The crucial region for aversion is located ventral and medial to the globus pallidus and dorsal and lateral to the lateral hypothalamus.