[Investigation on the process of sapindus saponin purified with macroporous adsorption resin and screening of its bacteriostasis].
[Investigation on the process of sapindus saponin purified with macroporous adsorption resin and screening of its bacteriostasis].
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发表时间：
2010-02
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通讯作者：
Yong Fu;P. Lei;Yu-mei Han;D. Yan
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作者：
Yong Fu;P. Lei;Yu-mei Han;D. Yan
目的研究大孔吸附树脂纯化黄芪皂苷的工艺参数。方法以皂苷洗脱率和纯化率为指标，考察了该工艺的吸附特性和洗脱参数。采用杯碟法对各洗脱部位的抑菌活性进行评价。结果无患子皂苷提取物13.6mL（生药量0.01g/mL）上大孔吸附树脂（phi 15 mm × H90 mm，干重2.5g），用3BV蒸馏水洗脱，再用3BV 30%乙醇和3BV 70%乙醇洗脱，大部分皂苷在70%乙醇中得到富集。结论采用大孔吸附树脂吸附纯化，皂苷洗脱率为93.8%，纯度为250.1%。因此，采用大孔吸附树脂吸附纯化皂苷的工艺是可行的，为其他类型皂苷的纯化提供了参考。
OBJECTIVE To study the technological parameters of the purification process of saponins with macroporous adsorption resin. METHODS The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of saponins as markers. Bacteriostasis activity of each parts eluted was evaluated by the mean of cup-plate method. RESULTS 13.6 mL of the extraction of sapindus saponin (crude drugs 0.01 g/mL) was purified with a column of macroporous adsorption resin (phi15 mm x H90 mm, dry weight 2.5 g) and washed with 3BV of distilled water, then eluted with 3BV of 30% ethanol and 3BV of 70% ethanol, most of saponins were collected in the 70% ethanol. CONCLUSION With macroporous adsorption resin adsorbing and purifying, the elutive ratio of saponins was 93.8% and the purity reached 250.1%. So this process of applying macroporous adsorption resin to adsorb and purify saponins is feasible, and supplies reference to the purification of other types of saponin.