Putranjivain A from Euphorbia jolkini inhibits both virus entry and late stage replication of herpes simplex virus type 2 in vitro
Putranjivain A from Euphorbia jolkini inhibits both virus entry and late stage replication of herpes simplex virus type 2 in vitro
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DOI：
10.1093/jac/dkh136
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发表时间：
2004-04-01
影响因子：
5.2
通讯作者：
Lin, CC
中科院分区：
文献类型：
--
作者：
Cheng, HY;Lin, TC;Lin, CC
目的：目的：研究从大戟科大戟属植物中分离得到的普坦吉万A（Putranjivain A）的体外抗病毒活性。使用XTT测定法显示，Putranjivain A表现出抗病毒活性，IC 50为7.9 +/-1.2 μ M，IC 50值随着感染复数的增加而增加。使用空斑减少试验，IC 50和IC 90分别为6.3 +/- 0.8和14.5 +/- 3.1 μ M。在达到抗病毒活性的浓度下，Putranjivain A对细胞增殖无细胞毒性作用。50%细胞毒性浓度（CC 50）为80.3 +/- 14.7 μ M，XTT和空斑减少试验的选择性指数（SI）（CC 50与IC 50的比值）分别为10.2和12.7。当测试杀病毒活性时，putranjivain A在75和100 μ M的浓度下显著降低病毒感染性，但在50 μ M或更低浓度下不降低。添加时间研究的结果表明，Putranjivain A在25 μ M时影响HSV-2复制的晚期。有趣的是，Putranjivain A也显示出对病毒附着和细胞渗透的抑制。结论：Putranjivain A具有抗病毒活性，能抑制病毒的粘附和穿透，并能干扰病毒后期的复制。
Aims: To investigate the in vitro antiviral properties of putranjivain A, isolated from the whole plant of Euphorbia jolkini Bioss (Euphorbiaceae).Methods and results: Herpes simplex virus (HSV)-2 strain 196-infected Vero cells were used. It was shown that putranjivain A exhibited antiviral activity with an IC50 of 7.9 +/- 1.2 muM using the XTT assay, with the IC50 value increasing with increasing multiplicity of infection. Using the plaque reduction assay, the IC50 and IC90 were 6.3 +/- 0.8 and 14.5 +/- 3.1 muM, respectively. Putranjivain A showed no cytotoxic effect on cell multiplication at concentrations that achieved antiviral activity. The 50% cell cytotoxic concentration (CC50) was 80.3 +/- 14.7 muM, and the selectivity index (SI) (ratio of CC50 to IC50) for the XTT and plaque reduction assays was 10.2 and 12.7, respectively. When tested for virucidal activity, putranjivain A significantly reduced viral infectivity at concentrations of 75 and 100 muM, but not at 50 muM or below. The results of the time-of-addition studies suggested that putranjivain A affected the late stage of HSV-2 replication at 25 muM. Interestingly, putranjivain A also showed inhibition of viral attachment and cell penetration. The combination of putranjivain A and aciclovir produced no interaction.Conclusions: Putranjivain A possesses antiviral activity, inhibiting viral attachment and penetration, and also interfering with the late stage of viral replication.