Molecular weight distribution and (1->3)(1->4)-beta-D-glucan content of consecutive extracts of various oat and barley cultivars
Molecular weight distribution and (1->3)(1->4)-beta-D-glucan content of consecutive extracts of various oat and barley cultivars
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DOI：
10.1094/cchem.1997.74.4.476
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发表时间：
1997-07-01
期刊：
影响因子：
2.4
通讯作者：
Weisz, J
中科院分区：
文献类型：
--
作者：
Beer, MU;Wood, PJ;Weisz, J
采用流动注射分析和高效粒径排除色谱法比较了不同燕麦和大麦品种提取物中β -葡聚糖的含量和分子量。结果表明，用含热稳定α -淀粉酶的热水（90℃）从燕麦和糯大麦中提取的β -葡聚糖含量为60 ~ 75%，而从非糯大麦中提取的β -葡聚糖含量仅为50 ~ 55%。用热水和二甲基亚砜（DMSO）连续提取总β -葡聚糖的65%（非糯大麦）或75-80%（燕麦和糯大麦）。氢氧化钠和硼氢化钠（NaOH/NaBH4）的萃取使β -葡聚糖提取率提高到86-100%，但降低了分子量。燕麦品种的β -葡聚糖分子量显著高于大麦品种。从非糯大麦品种中提取的β -葡聚糖的峰值分子量显著高于糯大麦品种。
The content and molecular weight (MW) of beta-glucan in extracts from a selection of oat and barley cultivars were compared using flow-injection analysis and high-performance size-exclusion chromatography From 60 to 75% of the beta-glucan was extracted from oat and waxy barley by hot water (90 degrees C) containing heat-stable alpha-amylase, whereas just 50-55% was extracted from nonwaxy barley. Consecutive extractions with hot water and dimethylsulfoxide (DMSO) extracted 65% (nonwaxy barley) or 75-80% (oat and waxy barley) of the total beta-glucan. An extraction with sodium hydroxide and sodium borohydride (NaOH/NaBH4) increased the percentage of beta-glucan extracted to 86-100% but decreased the MW. The MW of beta-glucan in the oat cultivars selected was significantly higher than those in the barley cultivars. The beta-glucan extracted from the nonwaxy barley cultivars showed significantly higher peak MW than that from the waxy barley cultivars.