Growth stimulation of human epidermal cells by urokinase is restricted to the intact active enzyme.
Growth stimulation of human epidermal cells by urokinase is restricted to the intact active enzyme.
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尿激酶对人表皮细胞生长的刺激仅限于完整的活性酶。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Bernd R. Binder
中科院分区：
文献类型：
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作者：
J. Kirchheimer;Guenter Christ;Bernd R. Binder
在这项研究中，我们报告了尿激酶片段对人表皮细胞系 CCL 20.2 细胞增殖的影响，CCL 20.2 表达尿激酶高亲和力受体，并且完整的活性 54-kDa 尿激酶刺激其生长。仅包含活性位点结构域的 33-kDa 片段不与受体结合，并且不刺激细胞增殖，而仅包含三环和生长因子结构域的 17-kDa 片段与受体结合，但不刺激人表皮细胞系的生长。因此，尿激酶对该肿瘤细胞系的生长促进仅限于完整且有活性的尿激酶分子。
In this study we report on the effect of urokinase fragments on the proliferation of cells of the human epidermal cell line, CCL 20.2, which expresses high-affinity receptors for urokinase and the growth of which is stimulated by intact active 54-kDa urokinase. The 33-kDa fragment containing only the active-site domain, does not bind to the receptors and does not stimulate cell proliferation, while the 17-kDa fragment, containing only the kringle and the growth-factor domains binds to the receptor but does not stimulate growth of the human epidermal cell line. Growth promotion of this tumor cell line by urokinase is therefore restricted to the complete intact and active urokinase molecule.