Saponin-enhanced biomass accumulation and demulsification capability of the demulsifying bacteria Alcaligenes sp S-XJ-1
Saponin-enhanced biomass accumulation and demulsification capability of the demulsifying bacteria Alcaligenes sp S-XJ-1
复制标题
皂苷增强破乳菌Alcaligenes sp S-XJ-1的生物量积累和破乳能力
DOI：
10.1039/c6ra02237e
复制
发表时间：
2016
期刊：
影响因子：
3.9
通讯作者：
Peng Kaiming
中科院分区：
文献类型：
--
作者：
Huang Xiangfeng;Zhang Yuyan;Wei Yansong;Liu Jia;Lu Lijun;Peng Kaiming
表面活性剂已广泛应用于疏水性有机物污染的生物修复。本研究以生物表面活性剂皂素为原料，以提高破乳菌Alcaligenes sp. S-XJ-1的生物量和破乳能力为目的，研究了生物表面活性剂皂素在提高菜籽油利用率中的作用。当皂素浓度为0.05%，接近其临界胶束浓度时，生物破乳剂的合成效果最佳，生物量和破乳率分别提高了2.4倍和18%。生物量的促进被揭示归因于皂苷增强利用菜籽油，从而改善油溶解，细胞膜渗透性和细胞活性。发现破乳增强与通过增加细胞表面的蛋白质含量和降低多糖含量的皂苷加强疏水性相关。这项研究提出了我们的理解，表面活性剂增强生物破乳剂的合成方面的HOC利用率和细胞表面组成的反应。
Surfactants have been widely applied to the bioremediation of hydrophobic organic compound (HOC) contamination. In this study, the biosurfactant saponin was used for improving rape oil utilization to increase the biomass and demulsification capability of the demulsifying bacterial strain Alcaligenes sp. S-XJ-1. A saponin concentration of 0.05%, near its critical micelle concentration, was optimal to enhance biodemulsifier synthesis; the biomass and demulsification ratio increased by 2.4-fold and 18%, respectively. Biomass promotion was revealed to be attributed to the saponin-enhanced utilization of rape oil resulting from improved oil solubilization, cell membrane permeability, and cell activity. The demulsification boost was found to correlate to saponin-strengthened hydrophobicity via an increased protein content and decreased polysaccharide content of the cell-surface. This study advances our understanding of surfactant-enhanced biodemulsifier synthesis with respect to HOC utilization and cell-surface composition responses.