Characterisation of porcine monocyte-derived dendritic cells according to their cytokine profile
Characterisation of porcine monocyte-derived dendritic cells according to their cytokine profile
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DOI：
10.1016/s0165-2427(02)00310-0
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发表时间：
2003-02-10
影响因子：
1.8
通讯作者：
Fossum, C
中科院分区：
文献类型：
--
作者：
Johansson, E;Domeika, K;Fossum, C
干扰素 (IFN)-α 对猪骨髓树突状细胞 (DC) 体外分化的影响通过 DC 在混合白细胞反应 (MLR) 中刺激细胞增殖的能力以及它们在暴露于细菌和病毒制剂时产生细胞因子的能力来评估。通过塑料粘附从纯化的外周血单核细胞 (PBMC) 中富集猪单核细胞，并在粒细胞巨噬细胞集落刺激因子 (GMCSF) 和白细胞介素 (IL)-4 或 GM-CSF、IL-4 和 IFN-α 存在的情况下培养。培养5天后，细胞形成树突状形态，并且通过流式细胞术测定，表达MHC II类和137分子的细胞比例增加。在 GM-CSF、IL-4 和 IFN-α 中分化 5 天的树突状细胞能够刺激同种异体和同种异体 PBMC 在 MLR 中增殖。 DC在仙台病毒刺激下产生Th1相关细胞因子IFN-α，并在质粒DNA（在脂转染存在下预孵育）、热灭活的胸膜肺炎放线杆菌、紫外线灭活的奥耶斯基氏病病毒和活仙台病毒刺激下产生IL-12。热灭活的细菌和仙台病毒还诱导产生 Th2 相关细胞因子 IL-10 和 IL-6。在GM-CSF和IL-4中的DC分化过程中添加IFN-α增强了它们刺激同种异体和同基因MLR的能力，但没有改变它们产生细胞因子的能力。 (C) 2003 Elsevier Science B.V. 保留所有权利。
The influence of interferon (IFN)-alpha on the in vitro differentiation of myeloid porcine dendritic cells (DC) was evaluated as the ability of the DC to stimulate to cell proliferation in a mixed leukocyte reaction (MLR), and as their ability to produce cytokines at exposure to bacterial and viral preparations. Porcine monocytes were enriched from purified peripheral blood mononuclear cells (PBMC) by plastic adherence and cultured in the presence of granulocyte-macrophage colony-stimulating factor (GMCSF) and interleukin (IL)-4 or in GM-CSF, IL-4 and IFN-alpha. After 5 days of culture, the cells developed a dendritic morphology and the proportion of cells expressing MHC class II and 137 molecules was increased as determined by flow cytometry. Dendritic cells, differentiated for 5 days in GM-CSF, IL-4 and IFN-alpha, were able to stimulate both allogeneic and syngeneic PBMC to proliferation in an MLR. The DC produced the Th1 associated cytokines IFN-alpha at Sendai virus stimulation, and IL-12 at stimulation with plasmid DNA (pre-incubated in the presence of lipofectin), heat-inactivated Actinobacillus pleuropneumoniae, UV-inactivated Aujeszky's disease virus and live Sendai virus. The heat-inactivated bacteria and Sendai virus also induced production of the Th2 associated cytokines IL-10 and IL-6. The addition of IFN-alpha during differentiation of DC in GM-CSF and IL-4 enhanced their ability to stimulate allogeneic and syngeneic MLR, but did not alter their ability to produce cytokines. (C) 2003 Elsevier Science B.V. All rights reserved.