OpenCell: A Low-cost, Open-Source, 3-in-1 device for DNA Extraction.
OpenCell: A Low-cost, Open-Source, 3-in-1 device for DNA Extraction.
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OpenCell：一种用于 DNA 提取的低成本、开源、三合一设备。
DOI：
10.1101/2023.09.18.558349
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Bhamla,MSaad
中科院分区：
文献类型：
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作者：
Gupta,Aryan;Yu,Justin;Challita,ElioJ;Standeven,Janet;Bhamla,MSaad
高成本的 DNA 提取程序给预算有限的实验室带来了巨大的挑战。为了解决这个问题，我们推出了 OpenCell，这是一种经济、开源、三合一实验室设备，结合了微珠均质器、微量离心机和涡旋混合器的功能。 OpenCell 采用模块化附件，通过磁性方式连接到中央旋转无刷电机。该电机与行星齿轮机构相连，可在一个紧凑的装置内实现高效的珠均质化、涡流混合和离心。 OpenCell 的设计融合了多种冗余安全功能，确保设备和操作员的安全。 RPM 测量、可编程定时器、电池供电和可选速度控制等附加功能使 OpenCell 成为可靠且可重复的实验室仪器。在我们的研究中，OpenCell 成功从菠菜（菠菜）中分离出 DNA，平均产量为 2.3 μg，A260/A280 比率为 1.77，证明了其对聚合酶链式反应 (PCR) 扩增等下游应用的有效性。 OpenCell 具有紧凑的尺寸（20 厘米 x 28 厘米 x 6.7 厘米）和轻量化设计（0.8 千克），与笔记本电脑的尺寸和重量相当，便于携带，使其成为中低收入国家资源有限环境和偏远野外站合成生物学的“背包实验室”的有吸引力的组成部分。利用 3D 打印和现成组件的可访问性，OpenCell 的制造和组装单位成本低于 50 美元，为成本超过 4000 美元的昂贵实验室设备提供了经济实惠的替代品。 OpenCell 旨在克服合成生物学研究的进入障碍，并为不断增加节俭和开放的硬件做出贡献。
High-cost DNA extraction procedures pose significant challenges for budget-constrained laboratories. To address this, we introduce OpenCell, an economical, open-source, 3-in-1 laboratory device that combines the functionalities of a bead homogenizer, a microcentrifuge, and a vortex mixer. OpenCell utilizes modular attachments that magnetically connect to a central rotating brushless motor. This motor couples to an epicyclic gearing mechanism, enabling efficient bead homogenization, vortex mixing, and centrifugation within one compact unit. OpenCell’s design incorporates multiple redundant safety features, ensuring both the device’s and operator’s safety. Additional features such as RPM measurement, programmable timers, battery operation, and optional speed control make OpenCell a reliable and reproducible laboratory instrument. In our study, OpenCell successfully isolated DNA fromSpinacia oleracea(spinach), with an average yield of 2.3 μg and an A260/A280 ratio of 1.77, demonstrating its effectiveness for downstream applications such as Polymerase Chain Reaction (PCR) amplification. With its compact size (20 cm x 28 cm x 6.7 cm) and lightweight design (0.8 kg), comparable to the size and weight of a laptop, OpenCell is portable, making it an attractive component of a ‘lab-in-a-backpack’ for resource-constrained environments in low-and-middle-income countries and synthetic biology in remote field stations. Leveraging the accessibility of 3D printing and off-the-shelf components, OpenCell can be manufactured and assembled at a low unit cost of less than $50, providing an affordable alternative to expensive laboratory equipment costing over $4000. OpenCell aims to overcome the barriers to entry in synthetic biology research and contribute to the growing collection of frugal and open hardware.