The efficacy of fluorocarbon, surfactant, and their combination for improving acute lung injury induced by intratracheal acidified infant formula
The efficacy of fluorocarbon, surfactant, and their combination for improving acute lung injury induced by intratracheal acidified infant formula
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DOI：
10.1213/01.ane.0000146438.87584.a9
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发表时间：
2005-04-01
影响因子：
5.7
通讯作者：
Obara, H
中科院分区：
文献类型：
--
作者：
Nishina, K;Mikawa, K;Obara, H
本研究旨在比较部分液体呼吸机(PLV)、肺表面活性物质(PSF)及其联合应用对酸化婴幼儿配方奶粉所致急性肺损伤(ALI)的改善作用。在第一部分研究中，42只接受呼气末正压为10cmH2O的容量控制通气组随机分为6组(无损伤I组、气体通气组[GVI]、PLVI组、PSFI组、PLVI->组、PSFI->组、PSFI->组；PLVI组)。采用气管内酸化婴幼儿配方奶粉(2mL/kg，pH 1.8)诱导大鼠ALI。GVI组既不接受PLV也不接受PSF治疗。PLV组和PSF组在酸化婴儿配方奶粉后30min分别气管内注入氟碳15mL/kg或表面活性物质100 mg/kg。PLVI-GT；PSFI组和PSFI->；PLVI组以30分钟为间隔接受两种治疗。在第二部分中，42只兔(共6组)接受与第一部分相同的药物治疗。在ALI诱导后150分钟，取肺组织进行生化和组织学检查。在第一部分和第二部分中，PSF、氟碳化合物及其组合可减轻肺内白细胞减少、中性粒细胞肿胀和超氧化物生成，从而改善氧合、肺力学和病理变化。与通风模式无关，PSF的效果最好，其次是氟碳，而氟碳的效果最差。
We conducted the current study to compare the efficacy of partial liquid ventilation (PLV), pulmonary surfactant (PSF), and their combination in ameliorating the acidified infant-formula-induced acute lung injury (ALI). In the Part I study, 42 rabbits receiving volume-controlled ventilation with positive end-expiratory pressure 10 cm H2O were randomly divided into 6 groups (groups noninjuryI, gas ventilation [GVI], PLVI, PSFI, PLVI -> PSFI, and PSFI -> PLVI). ALI was induced by intratracheal acidified infant formula (2 mL/kg, pH 1.8). Group GVI received neither PLV nor PSF therapy. Groups PLV and PSF received intratracheal fluorocarbon 15 mL/kg or surfactant 100 mg/kg, respectively, 30 min after acidified infant formula. Groups PLVI -> PSFI and PSFI -> PLVI received both treatments at 30-min intervals. In Part II, 42 rabbits (in 6 groups) undergoing pressure-controlled ventilation received the same drug therapies as in Part I. The lungs were excised to assess biochemical and histological damage 150 min after induction of ALI. In Parts I and II, PSF, fluorocarbon, and their combination attenuated lung leukosequestration and edema and superoxide production of neutrophils, consequently improving oxygenation, lung mechanics, and pathological changes. Independent of ventilation mode, PSF followed by fluorocarbon provided the most beneficial effects and fluorocarbon followed by PSF produced the least efficacy.