Comparative estrogenicity of estradiol, ethynyl estradiol and diethylstilbestrol in an in vivo, male sheepshead minnow (Cyprinodon variegatus), vitellogenin bioassay
Comparative estrogenicity of estradiol, ethynyl estradiol and diethylstilbestrol in an in vivo, male sheepshead minnow (Cyprinodon variegatus), vitellogenin bioassay
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DOI：
10.1016/s0166-445x(99)00076-4
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发表时间：
2000-05-01
影响因子：
4.5
通讯作者：
Denslow, ND
中科院分区：
文献类型：
--
作者：
Folmar, LC;Hemmer, M;Denslow, ND
开发了卵黄蛋白原（VTG）合成的体内生物测定法，以筛选单个化学物质或化学混合物在海洋硬骨鱼模型中的潜在雌激素作用。采用酶联免疫吸附测定 (ELISA) 来定量雄性羊头鲦鱼 (Cyprinodon variegatus) 暴露于五种浓度的天然雌激素 (17 β-雌二醇)、合成类固醇药物雌激素 (17 α-乙炔雌二醇) 或合成非类固醇药物雌激素 (己烯雌酚) 16 天，其 VTG 合成。在暴露浓度为 20 ng/l 时，只有二乙烯雌酚会引发卵黄生成反应。对于三种雌激素治疗，在所有大于 100 ng/l 的测试浓度下，VTG 以剂量依赖性方式出现在血浆中。还进行了肝脏 VTG mRNA 测量，结果显示数量之间没有明显的相关性，信息和成熟蛋白质的时间出现也不明显。这种测定短期相对便宜，显示直接反应，并且易于定量。 (C) 2000 Elsevier Science B.V. 保留所有权利。
An in vivo bioassay for vitellogenin (VTG) synthesis was developed to screen individual chemicals or mixtures of chemicals for potentially estrogenic effects in a marine teleost model. An enzyme-linked immunosorbent assay (ELISA) was used to quantitate VTG synthesis in male sheepshead minnows (Cyprinodon variegatus) exposed to five concentrations of the natural estrogen (17 beta-estradiol), a synthetic, steroidal pharmaceutical estrogen (17 alpha-ethynyl estradiol), or a synthetic, non-steroidal, pharmaceutical estrogen (diethylstilbestrol) for 16 days. At an exposure concentration of 20 ng/l, only diethystilbestrol elicited a vitellogenic response. At all test concentrations greater than 100 ng/l, VTG appeared in the plasma in a dose-dependent manner for the three estrogen treatments. Liver VTG mRNA measurements were also made, exhibiting no clear correlations between quantities, nor temporal appearance of the message and mature protein were apparent. This assay is short-term relatively inexpensive, shows a direct response, and easily quantitated. (C) 2000 Elsevier Science B.V. All rights reserved.