Microbial gene expression in Guaymas Basin subsurface sediments responds to hydrothermal stress and energy limitation
Microbial gene expression in Guaymas Basin subsurface sediments responds to hydrothermal stress and energy limitation
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DOI：
10.1038/s41396-023-01492-z
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发表时间：
2023-09-01
期刊：
影响因子：
11
通讯作者：
Edgcomb, Virginia
中科院分区：
文献类型：
--
作者：
Mara, Paraskevi;Zhou, Ying-Li;Edgcomb, Virginia
分析地下细菌和古生菌的基因表达有助于深入了解它们对地下原位条件的生理适应。我们研究了在墨西哥加利福尼亚州海湾瓜伊马斯盆地热液加热的海底沉积物中表达基因的模式，这些沉积物具有不同的地球化学和热机制。核糖核酸回收率和细胞计数随着沉积物深度的下降而下降，然而，我们从海底以下0.8m至101.9米深度的8个地点获得了转录本。我们描述了沉积物微生物的代谢潜力，并讨论了tRNA、mRNA和rRNA修饰中涉及的表达基因，这些修饰使细菌和古菌在热液地下具有生理灵活性。在受水热影响的环境中，如瓜伊马斯盆地，微生物分类群可能特别依赖这些催化RNA功能，因为它们调节细胞在高温和陡峭的地球化学梯度下的活动。还鉴定了与DNA修复、蛋白质维持和昼夜节律有关的表达基因。其中许多基因的协同作用可能对微生物在瓜伊马斯盆地地下生物圈中的生存和繁荣至关重要。
Analyses of gene expression of subsurface bacteria and archaea provide insights into their physiological adaptations to in situ subsurface conditions. We examined patterns of expressed genes in hydrothermally heated subseafloor sediments with distinct geochemical and thermal regimes in Guaymas Basin, Gulf of California, Mexico. RNA recovery and cell counts declined with sediment depth, however, we obtained metatranscriptomes from eight sites at depths spanning between 0.8 and 101.9 m below seafloor. We describe the metabolic potential of sediment microorganisms, and discuss expressed genes involved in tRNA, mRNA, and rRNA modifications that enable physiological flexibility of bacteria and archaea in the hydrothermal subsurface. Microbial taxa in hydrothermally influenced settings like Guaymas Basin may particularly depend on these catalytic RNA functions since they modulate the activity of cells under elevated temperatures and steep geochemical gradients. Expressed genes for DNA repair, protein maintenance and circadian rhythm were also identified. The concerted interaction of many of these genes may be crucial for microorganisms to survive and to thrive in the Guaymas Basin subsurface biosphere.