Neuroma: a donor-age independent source of human Schwann cells for tissue engineered nerve grafts
Neuroma: a donor-age independent source of human Schwann cells for tissue engineered nerve grafts
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DOI：
10.1097/00001756-200011270-00042
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发表时间：
2000-11-27
期刊：
影响因子：
1.7
通讯作者：
Wolf, G
中科院分区：
文献类型：
--
作者：
Keilhoff, G;Fansa, H;Wolf, G
雪旺细胞与生物基质结合使用，作为动物模型中的组织工程神经移植物，为治疗周围神经系统病变提供了新的治疗方法。从成人供体中获得高产量的人雪旺细胞仅通过药理学刺激实现，然而，在临床治疗中应避免。在这里，我们建立了激活的人雪旺细胞的培养物，这些细胞是从周围神经瘤中分离出来的，周围神经瘤是在正中神经损伤后发展起来的。为了使神经重建，神经瘤必须切除。这种神经瘤组织实际上是预变性的，并显示出许旺细胞的活化，这意味着良好的粘附和高有丝分裂活性。这使得无论供体年龄如何，都可以在短时间内生长，而无需任何药物治疗。NeuroReport 11：3805-3809（C）2000 Lippincott威廉姆斯和威尔金斯。
Schwann cells are used in combination with biological matrices as tissue engineered nerve grafts in animal models offering a new therapeutic approach for treatment of lesions of the peripheral nervous system.-A high yield of human Schwann cells from adult donors is only achieved by pharmacological stimulation, which should, however, be avoided in clinical therapy. Here, we establish cultures of activated human Schwann cells which were isolated from peripheral nerve neuroma which developed after a median nerve lesion. To allow nerve reconstruction neuroma have to be resected. Such neuroma tissue is virtually predegenerated and shows activation of Schwann cells, implying good adherence and high mitotic activity. This allows, irrespective of donor age, growing within a short time period and without any pharmacological treatment. NeuroReport 11:3805-3809 (C) 2000 Lippincott Williams & Wilkins.