Only fibres promoting a stable butyrate producing colonic ecosystem decrease the rate of aberrant crypt foci in rats
Only fibres promoting a stable butyrate producing colonic ecosystem decrease the rate of aberrant crypt foci in rats
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DOI：
10.1136/gut.48.1.53
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发表时间：
2001-01-01
期刊：
影响因子：
24.5
通讯作者：
Menanteau, J
中科院分区：
文献类型：
--
作者：
Perrin, P;Pierre, F;Menanteau, J
背景-膳食纤维被认为是预防结肠癌的保护剂，但流行病学和实验研究的结果都不确定。目的-假设对结肠癌的保护可能仅限于产生丁酸的纤维，我们调查了丁酸盐长期稳定产生所需的因素及其与结肠癌易感性的关系。方法-模仿人类的前瞻性研究，在大鼠身上进行了两部分随机盲法研究，使用低纤维控制饮食(CD)和三种高纤维饮食：无淀粉小麦麸皮(WB)、III型抗性淀粉(RS)和短链低聚果糖(FOS)。采用随机区组设计，96只近交系大鼠饲养2天、16天、30天或44天，以确定对饲料的适应期、发酵情况以及对结肠的影响，包括第44天的粘膜增殖。随后，36只饲喂相同饲料44天的大鼠被注射偶氮甲烷，并在30天后检查异常隐窝病灶。结果-发酵稳定后(44天)，只有RS和FOS产生大量丁酸，对大肠有营养作用。目前，不同饮食之间的粘膜增殖没有差异。在一个月后的后续实验中，喂食高丁酸饮食(RS，p=0.022；fos，p=0.043)的大鼠出现的异常隐窝病灶较少。结论-与所选纤维相关的稳定的丁酸产生结肠生态系统似乎不太利于结肠癌的发生。
Background-Dietary fibres have been proposed as protective agents against colon cancer but results of both epidemiological and experimental studies are inconclusive.Aims-Hypothesising that protection against colon cancer may be restricted to butyrate producing fibres, we investigated the factors needed for long term stable butyrate production and its relation to susceptibility to colon cancer.Methods-A two part randomised blinded study in rats, mimicking a prospective study in humans, was performed using a low fibre control diet (CD) and three high fibre diets: starch free wheat bran (WB), type III resistant starch (RS), and short chain fructo-oligosaccharides (FOS). Using a randomised block design, 96 inbred rats were fed for two, 16, 30, or 44 days to determine the period of adaptation to the diets, fermentation profiles, and effects on the colon, including mucosal proliferation on day 44. Subsequently, 36 rats fed the same diets for 44 days were injected with azoxymethane and checked for aberrant crypt foci 30 days later.Results-After fermentation had stabilised (44 days), only RS and FOS produced large amounts of butyrate, with a trophic effect in the large intestine. No difference in mucosal proliferation between the diets was noted at this time. In the subsequent experiment one month later, fewer aberrant crypt foci were present in rats fed high butyrate producing diets (RS, p=0.022; FOS, p=0.043),Conclusion-A stable butyrate producing colonic ecosystem related to selected fibres appears to be less conducive to colon carcinogenesis.