In vitro activity of ramoplanin and comparator drugs against anaerobic intestinal bacteria from the perspective of potential utility of pathology involving bowel flora
In vitro activity of ramoplanin and comparator drugs against anaerobic intestinal bacteria from the perspective of potential utility of pathology involving bowel flora
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DOI：
10.1016/j.anaerobe.2004.04.003
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发表时间：
2004-08-01
期刊：
影响因子：
2.3
通讯作者：
Wexler, HM
中科院分区：
文献类型：
--
作者：
Finegold, SM;St John, S;Wexler, HM
肠道细菌对各种体外抗菌药物的敏感性，以及这些药物在肠道内的水平，提供了药物对肠道菌群可能影响的信息。口服吸收不良的药物可能对治疗肠道感染和某些肠道细菌可能起作用的其他情况有用。采用nccls批准的Wadsworth布氏菌湖血琼脂稀释法测定了ramoplanin （MDL 62,198）对928株肠道厌氧菌的抑菌活性。比较ramoplanin与氨苄西林、杆菌肽、甲硝唑、甲氧苄啶/磺胺甲恶唑（TMP/SMX）、万古霉素的活性。检测的微生物包括脆弱拟杆菌群（89）、其他拟杆菌种（16）、其他革兰氏阴性厌氧杆状体（56）、厌氧球菌（114）、梭菌种（426）和不形成孢子的革兰氏阳性厌氧杆状体（227）。ramoplanin、氨苄西林、杆菌肽、甲硝唑和万古霉素的总MIC(90)s分别为256、32、128、16和128 mcg/ml。Ramoplanin对革兰氏阳性菌几乎总是高活性，而对革兰氏阴性菌，特别是拟杆菌、嗜杆菌、普雷沃氏菌和细孔菌的活性相对较差。万古霉素的活性与拉莫普兰相当。氨苄西林对经常产生β -内酰胺酶的生物体的活性相对较差，包括大多数革兰氏阴性杆状杆菌以及bolteae梭状芽孢杆菌和梭状芽孢杆菌。杆菌肽对大多数革兰氏阴性厌氧菌的活性相对较差，但对大多数革兰氏阳性菌的活性较好。甲硝唑对除双歧杆菌和部分非孢子形成革兰氏阳性杆菌外的所有菌群均有很好的抑制作用。TMP/SMX的活性非常差，MIC90为100 - 2048微克/毫升。(C) 2004 Elsevier Ltd.版权所有。
Susceptibility of intestinal bacteria to various antimicrobial agents in vitro, together with levels of those agents achieved in the gut, provides information on the likely impact of the agents on the intestinal flora. Orally administered drugs that are poorly absorbed may be useful for treatment of intestinal infections and for certain other situations in which intestinal bacteria may play a role. The antimicrobial activity of ramoplanin (MDL 62,198) against 928 strains of intestinal anaerobic bacteria was determined using the NCCLS-approved Wadsworth brucella laked-blood agar dilution method. The activity of ramoplanin was compared with that of ampicillin, bacitracin, metronidazole, trimethoprim/sulfamethoxazole (TMP/SMX), and vancomycin. The organisms tested included Bacteroides fragilis group (n = 89), other Bacteroides species (n = 16), other anaerobic Gram-negative rods (n = 56) anaerobic cocci (n = 114), Clostridium species (n = 426), and non-sporeforming anaerobic Gram-positive rods (n = 227). The overall MIC(90)s of ramoplanin, ampicillin, bacitracin, metronidazole, and vancomycin were 256, 32, 128, 16, and 128 mcg/ml, respectively.Ramoplanin was almost always highly active vs. Gram-positive organisms and relatively poor in activity against Gram-negative organisms, particularly Bacteroides, Bilophila, Prevotella, and Veillonella. Vancomycin was quite similar to ramoplanin in its activity. Ampicillin was relatively poor in activity vs. organisms that often produce beta-lactamase, including most of the Gram-negative rods as well as Clostridium bolteae and C clostridioforme. Bacitracin was relatively poor in activity against most anaerobic Gram-negative rods, but better vs. most Gram-positive organisms. Metronidazole was very active against all groups other than bifidobacteria and some strains of other types of non-sporeforming Gram-positive bacilli. TMP/SMX was very poorly active, with an MIC90 of > 2048 mcg/ml. (C) 2004 Elsevier Ltd. All rights reserved.