Isolation of 3,3'-neotrehalosadiamine (BMY-28251) from a butirosin-producing organism.
Isolation of 3,3'-neotrehalosadiamine (BMY-28251) from a butirosin-producing organism.
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从产生布替罗辛的生物体中分离 3,3-新海藻糖二胺 (BMY-28251)。
DOI：
10.7164/antibiotics.39.1346
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
H. Kawaguchi
中科院分区：
文献类型：
--
作者：
K. Numata;F. Satoh;M. Hatori;T. Miyaki;H. Kawaguchi
Sir：分离出肺炎克雷伯菌氨基糖苷类超敏突变体（Kp-126），用于新型氨基糖苷类抗生素的特异性检测'）。从布替罗辛生产菌株的发酵液中，检测到 3,3' 新海藻糖二胺 (BMY-28251) 作为一种对菌株 Kp-126 具有特异性活性的次要成分。该通讯描述了从环状芽孢杆菌 YQW-B62' 中分离和鉴定 BMY-28251。 BMY-28251 的生产使用含有可溶性淀粉 2%、葡萄糖 0.2%、豆粕 3%、MgSO、7H2O 0.3% 和 CaCO3 1% 的培养基 FR8。将生长良好的菌株 YQW-B6 的琼脂斜面接种到 500 ml 锥形瓶中的 100 ml 心脏浸液肉汤 (Difco) 中，并在旋转摇床 (180 rpm) 上于 32°C 培养 24 小时，以获得种子培养物。将2ml培养物转移至500ml烧瓶中的100ml培养基FR8中，在28℃下摇动4天。将肉汤滤液（2升）吸附到Amberlite IRC-50柱（70% NH4+形式）上。将柱用H 2 O洗涤，然后用1N NH 4 OH洗脱。将活性洗脱液合并并真空浓缩，得到作为总活性级分的吸湿性固体(2.8g)。将一部分固体（150mg）溶解在少量H2O中，并施加到Amberlite CG-50（NH+形式）柱（1.0cm×37cm）上。该柱以0.25nil/分钟的流速用H2O(级分编号1-10)、0.15N NH,OH(级分编号11-30)和0.5N NH,OH(级分编号31-80)逐步展开。使用Kp-126和枯草芽孢杆菌PCI 219 (Bs-1)作为测试微生物，通过纸盘琼脂扩散法监测每5ml洗脱液中的抗生素活性。如图 1 所示，峰 I（指定为组分 X）对 Kp-126 具有高活性，但对 Bs-1 活性较弱。其他两个峰显示出针对 Kp-126 和 Bs-1 的类似活性。巅峰III
Sir: The aminoglycoside hypersensitive mutant of Klebsiella pneumoniae (Kp-126) was isolated and used for a specific detection of new aminoglycoside-type antibiotics'). From the fermentation broth of a butirosin-producing strain, 3,3'neotrehalosadiamine (BMY-28251) was detected as a minor component specifically active against strain Kp-126. This communication describes the isolation and identification of BMY-28251 from Bacillus circulans YQW-B62'. The medium FR8 containing soluble starch 2 %, glucose 0.2 %, soybean meal 3 %, MgSO, • 7H2O 0.3 % and CaCO3 1 % was used for the production of BMY-28251. A well-grown agar slant of strain YQW-B6 was inoculated to 100 ml of the heart infusion broth (Difco) in 500-ml Erlenmeyer flask and incubated at 32°C for 24 hours on a rotary shaker (180 rpm) to obtain seed culture. Two ml of the culture was transferred into 100 ml of medium FR8 in 500-m1 flask which was shaken at 28°C for 4 days. The broth filtrate (2 liters) was adsorbed onto a column of Amberlite IRC-50 (70% NH4+ form). The column was washed with H2O and then eluted with I N NH4OH. Active eluates were combined and concentrated in vacuo to afford hygroscopic solid (2.8 g) as a total active fraction. A part of the solid (150 mg) was dissolved in a small amount of H,O and applied to a column (1.0 cm x 37 cm) of Amberlite CG-50 (NH,+ form). The column was developed stepwise at a flow rate of 0.25 nil/minute with H2O (fraction Nos. 1 10), 0.15 N NH,OH (fraction Nos. 11 30) and 0.5 N NH,OH (fraction Nos. 31 80). The antibiotic activity in each 5 ml of the elute was monitored by the paper disc-agar diffusion method using Kp-126 and Bacillus subtilis PCI 219 (Bs-1) as test organisms. As shown in Fig. 1, peak I, designated as component X, was highly active against Kp-126 but only weakly against Bs-1. The other two peaks showed comparable activity against Kp-126 and Bs-1. Peak III