Photo-induced degradation of some flavins in aqueous solution
Photo-induced degradation of some flavins in aqueous solution
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DOI：
10.1016/j.chemphys.2004.08.006
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发表时间：
2005-01-10
期刊：
影响因子：
2.3
通讯作者：
Hochmuth, E
中科院分区：
文献类型：
--
作者：
Holzer, W;Shirdel, J;Hochmuth, E
通过测量在428 nm处连续激发的吸收系数光谱变化，研究了pH为8的水溶液中FMN、FAD、核黄素、荧光黄素和光色胺在蓝光诱导下的光降解。测定的光降解量子产率为phi(D)（核黄素，pH 8）近似为7.8 x 10(-3), phi(D)（FMN, pH 5.6）近似为7.3 x 10(-3), phi(D)（FMN, pH 8）近似为4.6 x 10(-3), phi(D)（FAD, pH 8）近似为3.7 x 10(-4), phi(D)（光色素，pH 8）近似为1.8 x 10(-4), phi(D)（光黄素，pH 8）近似为1.1 x 10（-5）。在质谱分析中，FMN溶解在水中（溶液pH为5.6）的光产物被鉴定为光色胺和光黄素衍生物二羟甲基光黄素、甲酰基光黄素和光黄素-羟基乙醛。对FMN在pH值为8时的主要光产物进行了吸收和发射光谱表征。(C) 2004 Elsevier B.V.版权所有
The blue-light induced photo-degradation of FMN, FAD, riboflavin, lumiflavin, and lumichrome in aqueous solution at pH 8 is studied by measurement of absorption coefficient spectral changes due to continuous excitation at 428 nm. The quantum yields of photo-degradation determined are phi(D)(riboflavin, pH 8) approximate to 7.8 x 10(-3), phi(D)(FMN, pH 5.6) approximate to 7.3 x 10(-3), phi(D)(FMN, pH 8) approximate to 4.6 x 10(-3), phi(D)(FAD, pH 8) approximate to 3.7 x 10(-4), phi(D)(lumichrome, pH 8) approximate to 1.8 X 10(-4), and phi(D)(lumiflavin, pH 8) approximate to 1.1 X 10(-5). In a mass-spectroscopic analysis, the photo-products of FMN dissolved in water (solution pH is 5.6) were identified to be lumichrome and the lumiflavin derivatives dihydroxymethyllumiflavin, formyllumiflavin, and lumiflavin-hydroxy-acetaldehyde. An absorption and emission spectroscopic characterisation of the primary photoproducts of FMN at pH 8 is carried out. (C) 2004 Elsevier B.V. All rights reserved.