Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.
Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.
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DOI：
10.1016/j.molcel.2017.02.023
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发表时间：
2017-04-06
期刊：
影响因子：
16
通讯作者：
Winston F
中科院分区：
文献类型：
--
作者：
Shetty A;Kallgren SP;Demel C;Maier KC;Spatt D;Alver BH;Cramer P;Park PJ;Winston F
Spt5 is an essential and conserved factor that functions in transcription and co-transcriptional processes. However, many aspects of the requirement for Spt5 in transcription are poorly understood. We have analyzed the consequences of Spt5 depletion in Schizosaccharomyces pombe, using four genome-wide approaches. Our results demonstrate that Spt5 is crucial for a normal rate of RNA synthesis and distribution of RNAPII over transcription units. In the absence of Spt5, RNAPII localization changes dramatically, with reduced levels and a relative accumulation over the first ~500 bp, suggesting that Spt5 is required for transcription past a barrier. Spt5 depletion also results in widespread antisense transcription initiating within this barrier region. Deletions of this region alter the distribution of RNAPII on the sense strand, suggesting that the barrier observed after Spt5 depletion is normally a site at which Spt5 stimulates elongation. Our results reveal a global requirement for Spt5 in transcription elongation. Shetty et al. show that Spt5, a transcription factor conserved in all domains of life, globally regulates transcription by RNA polymerase II in vivo. Spt5 controls the level and rate of RNAPII transcription, its ability to elongate past a barrier, and the synthesis of distinct classes of antisense RNAs.
