Serum concentrations of 7 alpha-hydroxy-4-cholesten-3-one reflect bile acid synthesis in humans
Serum concentrations of 7 alpha-hydroxy-4-cholesten-3-one reflect bile acid synthesis in humans
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DOI：
10.1053/jhep.1996.v24.pm0008707250
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发表时间：
1996-07-01
期刊：
影响因子：
13.5
通讯作者：
Paumgartner, G
中科院分区：
文献类型：
--
作者：
Sauter, G;Berr, F;Paumgartner, G
7α-羟基-4-胆固酮-3-酮(α-HC)的血清浓度最近被证明可以反映人体胆固醇7α-羟基酶的活性。为了评价血清α-HC与胆汁酸合成的关系，同时测定了血清α-HC浓度和用气相色谱-质谱仪同位素稀释法测定的胆汁酸合成速率。回归分析表明，血清α-HC与胆酸(CA)(r=0.59，P=0.02)、鹅去氧胆酸(CDCA)(r=0.75)的合成呈线性正相关。P=.001)，两种初级胆汁酸的总合成量(r=.83，P<；在胆结石和肝功能正常的患者(n=15)中，α-HC也与脱氧胆酸(DCA)的投入率相关(r=.53，P<；.05)，慢性淤胆性肝病患者(n=5)的加入改善了血清α-HC与CA(r=.75，P&t；.001)、CDCA(r=.77，P&t；.001)以及两种初级胆汁酸的合成(r=.87，P<；；.001)，我们的数据与胆汁酸合成受胆固醇7α-羟基酶活性调节的概念一致，血清中的α-HC可作为半定量评估人类胆汁酸合成的方便标记物。
Serum concentrations of 7 alpha-hydroxy-4-cholesten-3-one (alpha-HC) have recently been shown to reflect the activity of cholesterol 7 alpha-hydroxylase in humans, To evaluate the relationship between alpha-HC in serum and bile acid synthesis, serum concentrations of alpha-HC, and rates of bile acid synthesis, as measured by the isotope dilution technique using gas chromatography-mass spectrometry, were determined simultaneously, Regression analysis revealed a positive linear correlation of serum alpha-Hc with synthesis of cholic acid (CA) (r = .59, P = .02), chenodeoxycholic acid (CDCA) (r = .75, P = .001), and total synthesis of both primary bile acids (r = .83, P < .001) in patients with gallstones and normal liver function (n = 15), alpha-HC was also correlated to input rates of deoxycholic acid (DCA) (r = .53, P < .05), Addition of patients with chronic cholestatic liver disease (n = 5) improved the correlation between serum alpha-HC and synthesis of CA (r = .75, P < .001), CDCA (r = .77, P < .001), and both primary bile acids combined (r = .87, P < .001), Our data are in agreement with the concept that synthesis of bile acids is regulated by cholesterol 7 alpha-hydroxylase activity and that alpha-HC in serum may serve as a convenient marker for the semiquantitative assessment of bile acid synthesis in humans.