Assays of polychlorinated biphenyl congeners and co-contaminated heavymetals in the transgenic Arabidopsis plants carrying the recombinant guinea pig aryl hydrocarbon receptor-mediated p-glucuronidase reportergene expression system
Assays of polychlorinated biphenyl congeners and co-contaminated heavymetals in the transgenic Arabidopsis plants carrying the recombinant guinea pig aryl hydrocarbon receptor-mediated p-glucuronidase reportergene expression system
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携带重组豚鼠芳基烃受体介导的β-葡萄糖醛酸酶报告基因表达系统的转基因拟南芥植物中多氯联苯同系物和共污染重金属的测定
DOI：
10.1080/03601234.2012.706549
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hideo Ohkawa and Hitoshi Ashida
中科院分区：
文献类型：
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作者：
Sayuri Shimazu;Masaya Ohta;Hideo Ohkawa and Hitoshi Ashida
采用重组豚鼠(g)芳烃受体（AhR）介导的β-葡萄糖醛酸酶（GUS）报告基因表达系统，对转基因拟南芥XgD2V11-6进行了多氯联苯（PCB）同源物和共污染重金属的检测。PCB126（毒性等效因子；TEF: 0.1）和PCB169 （TEF: 0.03）处理转基因拟南芥植株，GUS活性显著提高。经PCB80 （TEF: 0）处理后，GUS活性与0.1%二甲基亚砜（DMSO）处理后的GUS活性基本相同。PCB126与PCB169以1:1的比例混合后，GUS活性呈加性增加。然而，PCB126和PCB80混合暴露后，GUS活性低于单独处理PCB126。因此，PCB80似乎是AhR的拮抗剂。Fe、Cu、Zn、Cd、Pb和PCB126分别处理转基因植株时，Cd和Pb增加了PCB126诱导的GUS活性。另一方面，Fe、Cu和Zn对pcb126诱导的GUS活性没有影响。生物表面活性剂甘露糖红四醇脂质- b （MEL-B）和载体蛋白牛血清白蛋白（BSA）存在时，pcb126诱导的GUS活性增加，而cd辅助pcb126诱导的GUS活性不受影响。因此，MEL-B和BSA似乎分别增加了PCB126的摄取和转运。
The transgenicArabidopsisplant XgD2V11-6 carrying the recombinant guinea pig (g) aryl hydrocarbon receptor (AhR)-mediated β-glucuronidase (GUS) reporter gene expression system was examined for assay of polychlorinated biphenyl (PCB) congeners and co-contaminated heavy metals. When the transgenicArabidopsisplants were treated with PCB126 (toxic equivalency factor; TEF: 0.1) and PCB169 (TEF: 0.03), the GUS activity of the whole plants was increased significantly. After treatment with PCB80 (TEF: 0), the GUS activity was nearly the same level as that treated with 0.1% dimethylsulfoxide (DMSO) as a vehicle control. After exposure to a 1:1 mixture of PCB126 and PCB169, the GUS activity was increased additively. However, after exposure to a mixture of PCB126 and PCB80, the GUS activity was lower than that of the treatment with PCB126 alone. Thus, PCB80 seemed to be an antagonist towards AhR. When the transgenic plants were treated with each of the heavy metals Fe, Cu, Zn, Cd and Pb together with PCB126, Cd and Pb increased the PCB126-induced GUS activity. On the other hand, Fe, Cu and Zn did not affect the PCB126-induced GUS activity. In the presence of the biosurfactant mannosylerythritol lipid-B (MEL-B) and the carrier protein bovine serum albumin (BSA), the PCB126-induced GUS activity was increased, but the Cd-assisted PCB126-induced GUS activity was not affected. Thus, MEL-B and BSA seemed to increase uptake and transport of PCB126, respectively.