Regulation of the New Arabidopsis Imprinted Gene AtBMI1C Requires the Interplay of Different Epigenetic Mechanisms
Regulation of the New Arabidopsis Imprinted Gene AtBMI1C Requires the Interplay of Different Epigenetic Mechanisms
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DOI：
10.1093/mp/ssr078
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发表时间：
2012-01-01
期刊：
影响因子：
27.5
通讯作者：
Calonje, Myriam
中科院分区：
文献类型：
--
作者：
Bratzel, Fabian;Yang, Chao;Calonje, Myriam
最近，已经表明植物含有动物多梳抑制复合物1（PRC 1）组分BMI 1和RING 1A/B的同源物。在拟南芥中，有三个BMI 1样基因，其中两个，AtBMI 1A和B，需要在胚后植物生长过程中抑制胚胎性状，并允许细胞分化。然而，对第三个BMI 1样基因AtBMI 1C知之甚少。在这项工作中，我们发现AtBMI 1C只在胚乳和雄蕊发育过程中表达。AtBMI 1C是一个在胚乳中由母本等位基因表达但在雄蕊中双等位表达的印迹基因。我们发现AtBMI 1C的特征性表达模式是一个复杂的表观遗传调控的结果，涉及CG DNA甲基化，RNA指导的非CG DNA甲基化（RdDM）和PcG活性。我们的研究结果显示了不同的表观遗传机制在整个发育过程中调控基因表达的协调相互作用，揭示了目前植物胚乳印迹起源和机制的假设。
Recently, it has been shown that plants contain homologs to the animal Polycomb repressive complex 1 (PRC1) components BMI1 and RING1A/B. In Arabidopsis, there are three BMI1-like genes, two of which, AtBMI1A and B, are required during post-embryonic plant growth to repress embryonic traits and allow cell differentiation. However, little is known about the third BMI1-like gene, AtBMI1C. In this work, we show that AtBMI1C is only expressed during endosperm and stamen development. AtBMI1C is an imprinted gene expressed from the maternal allele in the endosperm but biallelically expressed in stamen. We found that the characteristic expression pattern of AtBMI1C is the result of a complex epigenetic regulation that involves CG DNA methylation, RNA-directed non-CG DNA methylation (RdDM), and PcG activity. Our results show the orchestrated interplay of different epigenetic mechanisms in regulating gene expression throughout development, shedding light on the current hypotheses for the origin and mechanism of imprinting in plant endosperm.