Engineering Cell-Compatible Paper Chips for Cell Culturing, Drug Screening, and Mass Spectrometric Sensing
Engineering Cell-Compatible Paper Chips for Cell Culturing, Drug Screening, and Mass Spectrometric Sensing
复制标题
用于细胞培养、药物筛选和质谱传感的工程细胞兼容纸芯片
DOI：
10.1002/adhm.201500383
复制
发表时间：
2015-10-28
影响因子：
10
通讯作者：
Lin, Jin-Ming
中科院分区：
文献类型：
--
作者：
Chen, Qiushui;He, Ziyi;Lin, Jin-Ming
纸支撑细胞培养是先进生物检测技术的一个前所未有的发展。这项研究报告了一种细胞兼容纸片的体外工程策略，该策略允许贴壁细胞培养、药物效率的定量评估以及通过纸喷雾质谱仪对细胞内分子的无标记传感。聚碳酸酯纸被用作细胞分布、黏附和生长的优秀替代生物支架，以及允许在没有光散射的情况下进行荧光成像。因此，细胞培养的纸片可以通过灵活的变形、堆叠和按细胞层组装来制造3D组织结构和共培养。因此，与细胞兼容的纸片的成功开发随后提供了一种独特的灵活方法，可以通过纸喷雾质谱仪原位检测活细胞成分，从而能够在最少的样品前处理即可分析细胞脂质和定量测量药物代谢。因此，所开发的用于贴壁细胞培养的纸片一次性使用价格低廉，与高通量兼容，并且易于免标记和快速分析。
Paper-supported cell culture is an unprecedented development for advanced bioassays. This study reports a strategy for in vitro engineering of cell-compatible paper chips that allow for adherent cell culture, quantitative assessment of drug efficiency, and label-free sensing of intracellular molecules via paper spray mass spectrometry. The polycarbonate paper is employed as an excellent alternative bioscaffold for cell distribution, adhesion, and growth, as well as allowing for fluorescence imaging without light scattering. The cell-cultured paper chips are thus amenable to fabricate 3D tissue construction and cocultures by flexible deformation, stacks and assembly by layers of cells. As a result, the successful development of cell-compatible paper chips subsequently offers a uniquely flexible approach for in situ sensing of live cell components by paper spray mass spectrometry, allowing profiling the cellular lipids and quantitative measurement of drug metabolism with minimum sample pretreatment. Consequently, the developed paper chips for adherent cell culture are inexpensive for one-time use, compatible with high throughputs, and amenable to label-free and rapid analysis.