Light-sheet microscopy using an Airy beam
Light-sheet microscopy using an Airy beam
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DOI：
10.1038/nmeth.2922
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发表时间：
2014-05-01
期刊：
影响因子：
48
通讯作者：
Dholakia, Kishan
中科院分区：
文献类型：
--
作者：
Vettenburg, Tom;Dalgarno, Heather I. C.;Dholakia, Kishan
光片显微镜有助于快速、高对比度、体积成像，样品曝光量最小。然而，传统高斯光片的快速发散限制了提供固有亚细胞分辨率的视场(FOV)。我们表明，艾里光束天生就能产生高对比度和分辨率，最高可达10倍大的视场。与贝塞尔光束不同的是，贝塞尔光束也提供了更大的视场，而艾里光束特有的不对称激发图案导致了所有对对比度有积极贡献的荧光，从而实现了光片显微镜的阶跃变化。
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian Light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.