Gene autoregulation by 3' UTR-derived bacterial small RNAs
Gene autoregulation by 3' UTR-derived bacterial small RNAs
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DOI：
10.7554/elife.58836
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发表时间：
2020-08-03
期刊：
影响因子：
7.7
通讯作者：
Papenfort, Kai
中科院分区：
文献类型：
--
作者：
Hoyos, Mona;Huber, Michaela;Papenfort, Kai
负反馈调节，即基因抑制自身合成的能力，是生物学已知的最丰富的调节基序。负反馈控制依赖于转录因子与其自身启动子的结合，这在转录调控中经常被报道。在这里，我们报告了一种新的机制，在细菌中的基因自动调节依赖于小调控RNA（sRNA）和主要的核糖核酸内切酶，RNase E。TIER-seq分析（瞬时失活核糖核酸内切酶，随后是RNA-seq）揭示了霍乱弧菌中类似的25，000个RNase E依赖性切割位点，其中一些导致稳定sRNA的积累。聚焦于两个实例，OppZ和CarZ，我们发现这些sRNA分别从oppABCDF和carAB操纵子的3'非翻译区（3' UTR）加工，并与它们自己的转录物碱基配对以抑制翻译。对于OppZ，该过程还触发Rho依赖性转录终止。我们的数据表明，来自3'UTR的sRNA作为自动调节元件，允许在转录后水平进行负反馈控制。
Negative feedback regulation, that is the ability of a gene to repress its own synthesis, is the most abundant regulatory motif known to biology. Frequently reported for transcriptional regulators, negative feedback control relies on binding of a transcription factor to its own promoter. Here, we report a novel mechanism for gene autoregulation in bacteria relying on small regulatory RNA (sRNA) and the major endoribonuclease, RNase E. TIER-seq analysis (transiently-inactivating-an-endoribonuclease-followed-by-RNA-seq) revealed similar to 25,000 RNase E-dependent cleavage sites in Vibrio cholerae, several of which resulted in the accumulation of stable sRNAs. Focusing on two examples, OppZ and CarZ, we discovered that these sRNAs are processed from the 3' untranslated region (3' UTR) of the oppABCDF and carAB operons, respectively, and basepair with their own transcripts to inhibit translation. For OppZ, this process also triggers Rho-dependent transcription termination. Our data show that sRNAs from 3' UTRs serve as autoregulatory elements allowing negative feedback control at the post-transcriptional level.