THE INFLUENCE OF PEPTIDES ON THE UPTAKE OF AMINO-ACIDS IN FUSOBACTERIUM - PREDICTED INTERACTIONS WITH PORPHYROMONAS-GINGIVALIS
THE INFLUENCE OF PEPTIDES ON THE UPTAKE OF AMINO-ACIDS IN FUSOBACTERIUM - PREDICTED INTERACTIONS WITH PORPHYROMONAS-GINGIVALIS
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DOI：
10.1007/bf01570167
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发表时间：
1989-10-01
影响因子：
2.6
通讯作者：
WELCH, SG
中科院分区：
文献类型：
--
作者：
GHARBIA, SE;SHAH, HN;WELCH, SG
氨基酸的摄取及其受肽源的影响的研究是以多变梭杆菌作为该属的方便代表进行的。参考菌株和临床分离株具有相似的氨基酸摄取曲线，但后者以较低浓度掺入大多数氨基酸。一般而言，所有种属均含有高水平的丝氨酸、天冬酰胺、谷氨酸、半胱氨酸和精氨酸。组氨酸、赖氨酸、苏氨酸和天冬氨酸的吸收水平较低，而非极性中性氨基酸如丙氨酸、缬氨酸、亮氨酸、异亮氨酸、甘氨酸、脯氨酸、苯丙氨酸和甲硫氨酸的代谢较差。酵母提取物，作为肽的来源，刺激了几种氨基酸，如组氨酸和谷氨酸的吸收，而其他如蛋氨酸，苏氨酸和天冬酰胺被抑制。一些氨基酸如天冬氨酸、鸟氨酸、赖氨酸和精氨酸的掺入不受肽的存在的影响。等摩尔氮浓度的氨基酸或氨不能取代肽的需求，强调肽作为能源的重要性。梭杆菌属的能力有限。在蛋白水解物种牙龈卟啉单胞菌的存在下，蛋白质的水解增加了约30%，并且可能代表了一种细菌相互作用，其中肽可能在体内被梭杆菌物种利用。
A study of the uptake of amino acids and its influence by a peptide source was a carried out with Fusobacterium varium as a convenient representative of the genus. Reference strains and a clinical isolate had similar amino acid uptake profiles, but most amino acids were incorporated at lower concentrations by the latter. In general, high levels of serine, asparagine, glutamate, cysteine, and arginine were incorporated by all species. Histidine, lysine, threonine, and aspartate were taken up at lower levels, whereas the nonpolar neutral amino acids such as alanine, valine, leucine, isoleucine, glycine, proline, phenylalanine, and methionine were poorly metabolized. Yeast extract, as a source of peptides, stimulated the uptake of several amino acids such as histidine and glutamate, whereas others such as methionine, threonine, and asparagine were repressed. The incorporation of some amino acids such as aspartate, ornithine, lysine, and arginine was unaffected by the presence of peptides. Equimolar nitrogen concentrations of amino acids or ammonia could not replace the peptide requirement, emphasizing the importance of peptides as an energy source. The limited capacity of Fusobacterium spp. to hydrolyze proteins increased approximately 30% in the presence of the proteolytic species, Porphyromonas gingivalis, and may represent one bacterial interaction in which peptides may become available to Fusobacterium species in vivo.