Effects of Food Lectins on the Transport System of Human Intestinal Caco-2 Cell Monolayers
Effects of Food Lectins on the Transport System of Human Intestinal Caco-2 Cell Monolayers
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DOI：
10.1271/bbb.130367
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发表时间：
2013-09-01
影响因子：
1.6
通讯作者：
Muramoto, Koji
中科院分区：
文献类型：
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作者：
Yamamoto, Shintaro;Tomiyama, Mai;Muramoto, Koji
应用4种荧光标记物：荧光黄(LY)代表细胞旁途径、荧光素(FL)代表单羧酸转运体途径、罗丹明123代表P-糖蛋白介导的外排途径和钙黄素代表多药耐药相关蛋白相关外排途径，研究了16种从食物中分离的凝集素对人肠道Caco-2细胞单层转运系统的影响。还测量了单层的跨上皮电阻(TER)值。来自小麦胚芽的WGA、来自白菇的ABA、来自米曲霉的AOL和来自鲑鱼卵的CSL3(每100微克/毫升)使TER值降低20-40%，从而增加了LY的转运。这些凝集素，以及其他凝集素，如大豆中的SBA、米糠中的RBA和菜豆中的ConA，也影响着其他的运输途径。这些结果表明，凝集素以不同的方式调节跨上皮运输系统，可能是因为它们与Caco-2细胞单层具有特异的结合特性。
The effects of 16 lectins isolated from foodstuff on the transport system across human intestinal Caco-2 cell monolayers were investigated by using four fluorescent markers: lucifer yellow (LY) for the paracellular pathway, fluorescein (FL) for the monocarboxylic acid transporter-mediated pathway, rhodamine 123 for the P-glycoprotein-mediated efflux pathway, and calcein for the multidrug resistance associated protein-related efflux pathway. The transepithelial electrical resistance (TER) values for the monolayers were also measured. WGA from wheat germ, ABA from white mushroom, AOL from Aspergillus oryzae, and CSL3 from chum salmon eggs (each at 100 mu g/mL) decreased the TER value by 20-40% which resulted in increased LY transport. These lectins, as well as such other lectins as SBA from soybean, RBA from rice bran, and Con A from jack bean, affected other transport pathways too. These results indicate that the lectins modulated the transepithelial transport system in different ways, probably because of their specific binding characteristics toward Caco-2 cell monolayers.