Comparative transcriptome analysis of male and female gonads reveals sex-biased genes in spotted scat (Scatophagus argus)
Comparative transcriptome analysis of male and female gonads reveals sex-biased genes in spotted scat (Scatophagus argus)
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雄性和雌性性腺的比较转录组分析揭示了斑点粪便（Scatophagus argus）中的性别偏见基因
DOI：
10.1007/s10695-019-00693-8
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发表时间：
2019-12-01
影响因子：
2.9
通讯作者：
Zhu, Chun-Hua
中科院分区：
文献类型：
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作者：
He, Fei-Xiang;Jiang, Dong-Neng;Zhu, Chun-Hua
乌鳢是东亚和东南亚新兴的水产养殖鱼类。迄今为止，S.生殖发育和调控的研究。缺乏争论。此外，还缺乏有关生殖的遗传和基因组信息，例如性腺转录组数据。在此，我们报告了 S 的第一个性腺转录组。 argusand 识别出可能参与生殖和性腺发育的基因。通过对III期睾丸（n=≥3）和卵巢（n=≥3）进行测序，总共获得了136,561个unigenes。鉴定出已知参与性腺发育和配子发生的男性和女性上调的基因，包括男性偏向的ddmrt1、amh、gsdf、wt1a、sox9b和nanos2，以及女性偏向的foxl2、gdf9、bmp15、sox3、zar1和figla。雌性和雄性鱼的血清雌二醇-17β和11-酮睾酮水平分别存在偏差。通过对 cyp19a1a 和 cyp11b2 等 20 个类固醇生成相关基因的表达分析，解释了血清类固醇激素水平的性别二态性。该性腺转录本数据集将有助于研究与生殖相关的功能基因。阿格斯。
Scatophagus argusis a new emerging aquaculture fish in East and Southeast Asia. To date, research on reproductive development and regulation inS. argusis lacking. Additionally, genetic and genomic information about reproduction, such as gonadal transcriptome data, is also lacking. Herein, we report the first gonadal transcriptomes ofS. argusand identify genes potentially involved in reproduction and gonadal development. A total of 136,561 unigenes were obtained by sequencing of testes (n= 3) and ovaries (n= 3) at stage III. Genes upregulated in males and females known to be involved in gonadal development and gametogenesis were identified, including male-biaseddmrt1,amh,gsdf,wt1a,sox9b, andnanos2, and female-biasedfoxl2,gdf9,bmp15,sox3,zar1, andfigla. Serum estradiol-17β and 11-ketotestosterone levels were biased in female and male fish, respectively. Sexual dimorphism of serum steroid hormone levels were interpreted after expression analysis of 20 steroidogenesis-related genes, includingcyp19a1aandcyp11b2. This gonadal transcript dataset will help investigate functional genes related to reproduction inS. argus.