Carbon nanodots as molecular scaffolds for development of antimicrobial agents.
Carbon nanodots as molecular scaffolds for development of antimicrobial agents.
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DOI：
10.1016/j.bmcl.2016.02.047
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发表时间：
2016-04-01
影响因子：
2.7
通讯作者：
Hargrove A
中科院分区：
文献类型：
--
作者：
Ngu-Schwemlein M;Chin SF;Hileman R;Drozdowski C;Upchurch C;Hargrove A
我们报道了碳纳米点（CND）作为分子支架用于增强小树枝状聚酰胺胺（PAMAM）的抗菌活性的潜力。以西米淀粉为原料制备的碳纳米点，通过N-乙基-N′-（3-二甲氨基丙基）碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）与PAMAM进行功能化。这些聚胺化CND的电子显微镜图像显示它们的直径约为30-60 nm。水溶性材料的红外和荧光光谱分析确定了聚酰胺胺化部分的存在和纳米点的固有荧光。聚胺化纳米点（CND-PAM 1和CND-PAM 2）表现出体外抗菌性能，不仅对非多药耐药细菌，而且对相应的革兰氏阴性多药细菌。它们的最小抑菌浓度（MIC）范围为8至64 µg/mL，远低于PAMAM G1或无活性PAMAM G 0和CND。此外，它们与四环素或粘菌素组合显示协同作用。这些初步结果表明，CND可以作为一个有前途的支架，促进抗菌材料的合理设计，以应对不断增加的抗生素耐药性的威胁。此外，它们的荧光可能与揭示其成像或诊断应用的作用模式有关。
We report the potential of carbon nanodots (CNDs) as a molecular scaffold for enhancing the antimicrobial activities of small dendritic poly(amidoamines) (PAMAM). Carbon nanodots prepared from sago starch are readily functionalized with PAMAM by using N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Electron microscopy images of these polyaminated CNDs show that they are approximately 30–60 nm in diameter. Infrared and fluorescence spectroscopy analyses of the water-soluble material established the presence of the polyamidoaminated moiety and the intrinsic fluorescence of the nanodots. The polyaminated nanodots (CND-PAM1 and CND-PAM2) exhibit in vitro antimicrobial properties, not only to nonmultidrug resistant bacteria but also to the corresponding Gram-negative multidrug bacteria. Their minimum inhibitory concentration (MIC) ranges from 8 to 64 µg/mL, which is much lower than that of PAMAM G1 or the non-active PAMAM G0 and CNDs. Additionally, they show synergistic effect in combination with tetracycline or colistin. These preliminary results imply that CNDs can serve as a promising scaffold for facilitating the rational design of antimicrobial materials for combating the ever-increasing threat of antibiotic resistance. Moreover, their fluorescence could be pertinent to unraveling their mode of action for imaging or diagnostic applications.