Variability of core microbiota in newly diagnosed treatment-naive paediatric inflammatory bowel disease patients
Variability of core microbiota in newly diagnosed treatment-naive paediatric inflammatory bowel disease patients
复制标题
DOI：
10.1371/journal.pone.0197649
复制
发表时间：
2018-08-13
期刊：
影响因子：
3.7
通讯作者：
Budding, A. E.
中科院分区：
文献类型：
--
作者：
de Meij, T. G. J.;de Groot, E. F. J.;Budding, A. E.
背景与目的肠道菌群被认为在炎症性肠病（IBD）的病因学中起着至关重要的作用。我们的目的是描述粪便微生物群组成和动力学在一个大的队列的儿童与新发（幼稚）IBD，在比较健康的儿科对照（HC）。方法在这项前瞻性研究中，在两个三级中心进行，粪便样本从新诊断，治疗幼稚的儿科IBD患者收集肠道清洗前的结肠镜检查（t0）和1，治疗开始后3周、6周和3个月。克罗恩病（CD）和溃疡性结肠炎（UC）患者的微生物谱与HC进行了比较，并与治疗反应相关。微生物群组成进行了分析，IS-亲technology.Results104新IBD患者（63 CD，41 UC，中位年龄14.0岁）的微生物概况进行了比较，61 HC（中位数7.8年）。IBD的主要特征是Alistipes finegoldii和Alistipes putredinis丰度降低，这是健康状态微生物核心的特征。包括这些核心物种作为预测因子的分类器实现了0.87的ROC曲线的AUC。核心细菌往往在治疗过程中恢复丰富，但没有达到健康的level.ConclusionFaecal微生物群配置文件的儿童从头CD和UC可以区分HC具有很高的准确性，主要是由一个减少的丰度的物种塑造的微生物的核心在健康状态。因此，儿科IBD的特征在于反映健康状态的某些细菌种类的丰度降低，而不是病原体的引入。
Background & aimsIntestinal microbiota is considered to play a crucial role in the aetiology of inflammatory bowel disease (IBD). We aimed to describe faecal microbiota composition and dynamics in a large cohort of children with de novo (naive) IBD, in comparison to healthy paediatric controls (HC).MethodsIn this prospective study, performed at two tertiary centres, faecal samples from newly diagnosed, treatment-naive paediatric IBD patients were collected prior to bowel cleansing for colonoscopy (t0) and 1, 3 and 6 weeks and 3 months after initiation of therapy. The microbial profiles of Crohn's disease (CD) and Ulcerative colitis (UC) patients were compared with HC and linked to therapeutic response. Microbiota composition was analysed by IS-pro technology.ResultsMicrobial profiles of 104 new IBD-patients (63 CD, 41 UC, median age 14.0 years) were compared to 61 HC (median 7.8 years). IBD was mainly characterised by decreased abundance of Alistipes finegoldii and Alistipes putredinis, which characterize a healthy state microbial core. The classifier including these core species as predictors achieved an AUC of the ROC curve of .87. Core bacteria tended to regain abundance during treatment, but did not reach healthy levels.ConclusionFaecal microbiota profiles of children with de novo CD and UC can be discriminated from HC with high accuracy, mainly driven by a decreased abundance of species shaping the microbial core in the healthy state. Paediatric IBD can therefore be characterized by decreased abundance of certain bacterial species reflecting the healthy state rather than by the introduction of pathogens.