Antigen presentation by a macrophage-like cell line persistently infected with respiratory syncytial virus
Antigen presentation by a macrophage-like cell line persistently infected with respiratory syncytial virus
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DOI：
10.1016/j.virusres.2003.10.005
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发表时间：
2004-01-01
期刊：
影响因子：
5
通讯作者：
Gómez, B
中科院分区：
文献类型：
--
作者：
Guerrero-Plata, A;Ortega, E;Gómez, B
生命早期人类呼吸道合胞病毒（RSV）的严重感染与随后的复发性气道疾病有关，可能是由局部免疫反应失调介导的。免疫反应功能障碍可能与巨噬细胞功能受损有关。我们之前曾报道过，RSV 在巨噬细胞培养物 (MPhiDper) 中的持续存在会改变 Fcy 受体 (Fcgamma) 介导的吞噬作用和促炎细胞因子的产生。在此，我们确定了M(Dper中巨噬细胞处理和呈递抗原以及刺激RSV特异性CD8(+)T细胞的能力是否发生改变。我们还检测了M(Dper中MHC I类分子的表达水平以及这些细胞向特定T淋巴细胞呈递病毒抗原的能力。我们的结果表明，抗原加工和呈递并未因慢性RSV感染而改变，并表明M(Dper能够刺激RSV特异性CD8(+)T淋巴细胞。 (C) 2003 Elsevier B.V. 保留所有权利。
Severe infection by the human respiratory syncytial virus (RSV) early in life is associated with subsequent recurrent airway disease presumably mediated by dysregulation of the local immune response. Dysfunction of the immune response may be related to impaired macrophage functions. We have previously reported that RSV persistence in a macrophage culture (MPhiDper) alters Fcy receptors (Fcgamma) -mediated phagocytosis and the production of pro-inflammatory cytokines. Here, we determined whether the ability of macrophages to process and present antigens and to stimulate RSV-specific CD8(+) T cells was altered in M(Dper. We also examined the level of expression of MHC class I molecules in M(Dper and the ability of these cells to present viral antigens to specific T lymphocytes. Our results showed that antigen processing and presentation were not altered by chronic RSV infection, and suggested that M(Dper were able to stimulate RSV-specific CD8(+) T lymphocytes. (C) 2003 Elsevier B.V. All rights reserved.