Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction
Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction
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DOI：
10.1016/s0015-0282(97)00278-1
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发表时间：
1997-10-01
影响因子：
6.7
通讯作者：
Seifer, DB
中科院分区：
文献类型：
--
作者：
Friedman, CI;Danforth, DR;Seifer, DB
目的：确定育龄晚期妇女的卵泡液（FF）是否相对缺乏血管生成细胞因子血管内皮生长因子/血管通透性因子。此外，我们试图确定是否黄体化颗粒细胞分泌血管内皮生长因子/血管通透性因子在应对hypoxia.Design：回顾性队列研究。设置：大学教学hospital.Patients：妇女接受卵泡抽吸超排卵后准备体外受精-胚胎移植。晚期育龄妇女包括21名大于或等于38岁（范围，38至46岁）的妇女; 15名小于或等于30岁的受试者作为对照population.Intervention（s）：在hCG后35小时通过经阴道抽吸收集颗粒细胞和FF。将来自两名妇女的颗粒细胞在M199 + 10%胎牛血清中在1%O-2- 5%CO2 - 94%N-2（缺氧）或95%空气-5%CO2中培养24和48小时（常氧）不含或含0.1 mol/L氯化钴。主要观察指标：合并的FF血管内皮生长因子/血管通透性因子浓度和培养基血管内皮生长因子/测定24和48小时的血管通透性因子累积。卵泡液中血管内皮生长因子/血管渗透因子浓度在高龄育龄妇女中高于年轻妇女（3，735 ± 2，155 vs 2，205 ± 952 pg/mL，平均值± SD）。在5% CO2和空气中维持的培养基中，24和48小时时血管内皮生长因子/血管通透性因子的蓄积分别为391 +/- 54和744 +/- 2 pg/mL。氯化钴诱导血管内皮生长因子/血管通透性因子显著增加（24小时为2，008 +/- 52 pg/mL，48小时为3，630 +/- 519 pg/mL）。血管内皮生长因子/血管通透性因子中度但显著增加与常氧对照组相比，在1%O-2条件下观察到的浓度（24小时为733 +/- 35 pg/mL，48小时为2，675 +/- 864 pg/mL）。在给予hMG和hCG后，晚期育龄妇女的FF显示血管内皮生长因子/血管通透性因子浓度与年轻女性相比。血管内皮生长因子/血管通透性因子浓度增加可能与老年妇女卵泡内的缺氧环境一致。(C)1997年，美国生殖医学会。
Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia.Design: Retrospective cohort study.Setting: University teaching hospital.Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women greater than or equal to 38 years old (range, 38 to 46 years); 15 subjects less than or equal to 30 years served as the control population.Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10% fetal bovine serum in 1% O-2-5% CO2-94% N-2 (hypoxic) or 95% air-5% CO2 (normoxic) without or with 0.1 mol/L cobalt chloride.Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined.Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 +/- 2,155 versus 2,205 +/- 952 pg/mL, mean +/- SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 +/- 54 and 744 +/- 2 pg/mL in media maintained in 5% CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 +/- 52 pg/mL at 24 hours and 3,630 +/- 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 +/- 35 pg/mL at 24 hours and 2,675 +/- 864 pg/mL at 48 hours) was observed with 1% O-2 compared with normoxic controls.Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women. (C) 1997 by American Society for Reproductive Medicine.