The effect of burn injury on CD8+ and CD4+ T cells in an irradiation model of homeostatic proliferation.
The effect of burn injury on CD8+ and CD4+ T cells in an irradiation model of homeostatic proliferation.
复制标题
稳态增殖辐射模型中烧伤对 CD8 和 CD4 T 细胞的影响。
DOI：
10.1097/01.ta.0000195984.56153.21
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Cairns,BruceA
中科院分区：
文献类型：
--
作者：
Buchanan,IanB;Maile,Robert;Frelinger,JeffreyA;Fair,JeffreyH;Meyer,AnthonyA;Cairns,BruceA
Background:Homeostatic proliferation of T cells has recently been shown to be an important mechanism in the host response to infection. However, its role in the T cell response to burn injury is unknown. In this study, we examine the effect of burn injury on CD4+ and CD8+ T cell homeostatic proliferation after irradiation.Methods:Wild-type C57BL/6 female mice were irradiated with six grays ionizing radiation and 48 hours later, syngeneic whole splenocytes or purified CD4+ or CD8+ T cells labeled with carboxy-fluorescein diacetate, succinimidyl ester were adoptively transferred. Two days later, mice underwent a 20% burn injury, followed by splenocyte harvest 3 and 10 days after injury.Results:Burn mice demonstrate increased splenic cellularity and CD8+ T cell proliferation after adoptive transfer of either purified CD8+ cells or whole spleen populations compared with unburned (sham) mice. In contrast, CD4+ T cell proliferation after burn injury is unchanged after adoptive transfer of whole spleen cells and drastically decreased after adoptive transfer of a purified CD4+ population compared with sham mice. Ten days after burn injury CD8+ T cells continue to demonstrate greater proliferation than CD4+ T cells.Conclusions:CD8+ T cells are more robust than CD4+ T cells in their proliferative response after burn injury. In addition, CD8+ T cell proliferation appears less reliant on other immune cells than purified CD4+ T cell proliferation. These data reiterate the importance of CD8+ T cells in the initial immune response to burn injury.
