ESTIMATION OF ELUTION TIMES ON REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PYRIDYLAMINO DERIVATIVES OF SUGAR CHAINS FROM GLYCOPROTEINS
ESTIMATION OF ELUTION TIMES ON REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PYRIDYLAMINO DERIVATIVES OF SUGAR CHAINS FROM GLYCOPROTEINS
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DOI：
10.1016/0003-2697(90)90025-5
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发表时间：
1990-01-01
影响因子：
2.9
通讯作者：
IKENAKA, T
中科院分区：
文献类型：
--
作者：
HASE, S;IKENAKA, T
在吡啶氨基（PA）糖链上添加糖残基会影响其在反相HPLC上的洗脱时间，并且糖残基的贡献不受其他糖残基的影响[S. Hase，S. Natsuka，H. Oku和T. 03 The Dog（1987）167，321-326]。从三种PA-糖链的相对洗脱时间（相对于Man 5Glc-NAc 2-PA的洗脱时间）计算22个糖残基的部分相对洗脱时间（Ei）：寡甘露糖和N-乙酰乳糖胺类型和具有木糖残基的糖链。PA-糖链的相对洗脱时间可以通过将所有组成糖残基的Ei值相加来计算。44个PA-糖链的相对洗脱时间的计算值与实测值吻合良好。该方法可用于估计PA-糖链的相对洗脱时间，这些时间尚不可用，并且通过糖组成分析和外切糖苷酶消化的组合来估计有限量的糖蛋白中的糖链的结构。
Addition of a sugar residue to a pyridylamino (PA) sugar chain affects its elution time on reverse-phase HPLC and the contribution of the sugar residue is not influenced by the other sugar residues [S. Hase, S. Natsuka, H. Oku, and T. Ikenaka (1987) Anal. Biochem. 167, 321-326]. The partial relative elution times (Ei) of 22 sugar residues were calculated from the relative elution times (the elution time relative to Man5Glc-NAc2-PA) of three kinds of PA-sugar chain: the oligomannose and N-acetyllactosamine types and sugar chains with a xylose residue. The relative elution time of a PA-sugar chain can be calculated by summing the Ei values of all of the constituent sugar residues. The calculated relative elution times of 44 PA-sugar chains agreed well with the observed values. This method can be used to estimate the relative elution times of PA-sugar chains that are not yet available, and to estimate the structures of sugar chains in limited amounts of glycoproteins by a combination of sugar composition analysis and exoglycosidase digestion.