MONOCYTE-MACROPHAGE DIFFERENTIATION IN EARLY MULTIPLE-SCLEROSIS LESIONS
MONOCYTE-MACROPHAGE DIFFERENTIATION IN EARLY MULTIPLE-SCLEROSIS LESIONS
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DOI：
10.1002/ana.410380514
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发表时间：
1995-11-01
影响因子：
11.2
通讯作者：
LASSMANN, H
中科院分区：
文献类型：
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作者：
BRUCK, W;PORADA, P;LASSMANN, H
在疾病早期获得的多发性硬化症（MS）病变的活检样本中研究了单核细胞/巨噬细胞的分化。使用一组识别不同巨噬细胞激活抗原的抗体通过免疫细胞化学来鉴定巨噬细胞。用每种抗体染色的细胞数量与通过髓磷脂降解产物的存在检测到的病变的脱髓鞘活性相关。全巨噬细胞标记物 Ki-M1P 显示早期和晚期活动性病变中巨噬细胞数量最多。在非活动性、脱髓鞘或髓鞘再生病变中遇到的数量较少。急性期炎症巨噬细胞标记物 MRP14 和 27E10 仅在早期活动性 (MRP14) 或早期和晚期活动性 (27E10) 病变中表达，从而可以识别活动性脱髓鞘病变。相反，慢性阶段炎症巨噬细胞标记物25F9随着病变活动的降低而表现出表达增加。这些发现表明多发性硬化症病变中巨噬细胞激活的差异模式，并允许在常规固定和石蜡包埋的组织中对脱髓鞘病变进行分期。
Monocyte/macrophage differentiation was studied in biopsy samples of multiple sclerosis (MS) lesions obtained in the early course of the disease. Macrophages were identified by immunocytochemistry using a panel of antibodies recognizing different macrophage-activation antigens. The number of cells stained with each antibody was related to the demyelinating activity of the lesions as detected by the presence of myelin degradation products. The pan-macrophage marker Ki-M1P revealed the highest numbers of macrophages in early and late active lesions. Lower numbers were encountered in inactive, demyelinated, or remyelinated lesions. The acute stage inflammatory macrophage markers MRP14 and 27E10 were expressed in either only early active (MRP14) or early and late active (27E10) lesions, thus allowing the identification of actively demyelinating lesions. The chronic stage inflammatory macrophage marker 25F9, in contrast, showed increasing expression with decreasing lesional activity. These findings indicate a differentiated pattern of macrophage activation in MS lesions and allow the staging of demyelinating lesions in routinely fixed and paraffin-embedded tissue.